A patatin‐like phospholipase functions during gametocyte induction in the malaria parasite Plasmodium falciparum

Autor: Jude M Przyborski, Yoshiki Yamaryo-Botté, Philipp Aurass, Cyrille Y. Botté, Gabriele Pradel, Ansgar Flammersfeld, Antje Flieger, Atscharah Panyot
Přispěvatelé: Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Robert Koch Institute [Berlin] (RKI)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Cytoplasm
Plasmodium falciparum
Immunology
Protozoan Proteins
[SDV.CAN]Life Sciences [q-bio]/Cancer
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Biology
Phospholipase
Microbiology
Mice
03 medical and health sciences
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Virology
ddc:570
medicine
Gametocyte
Animals
Parasite hosting
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Gene
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
Life Cycle Stages
0303 health sciences
030306 microbiology
Lipid Metabolism
medicine.disease
biology.organism_classification
[SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM]
Recombinant Proteins
3. Good health
Cell biology
Gene Expression Regulation
Patatin-like phospholipase
Phospholipases
Gene Knockdown Techniques
Female
Genome
Protozoan
Malaria
Zdroj: Cellular Microbiology
Cellular Microbiology, Wiley, 2019, ⟨10.1111/cmi.13146⟩
Cellular microbiology 22(3), e13146 (2020). doi:10.1111/cmi.13146
ISSN: 1462-5814
1462-5822
Popis: Patatin-like phospholipases (PNPLAs) are highly conserved enzymes of prokaryotic and eukaryotic organisms with major roles in lipid homeostasis. The genome of the malaria parasite Plasmodium falciparum encodes four putative PNPLAs with predicted functions during phospholipid degradation. We here investigated the role of one of the plasmodial PNPLAs, a putative PLA2 termed PNPLA1, during blood stage replication and gametocyte development. PNPLA1 is present in the asexual and sexual blood stages and here localizes to the cytoplasm. PNPLA1-deficiency due to gene disruption or conditional gene-knockdown had no effect on intraerythrocytic growth, gametocyte development and gametogenesis. However, parasites lacking PNPLA1 were impaired in gametocyte induction, while PNPLA1 overexpression promotes gametocyte formation. The loss of PNPLA1 further leads to transcriptional down-regulation of genes related to gametocytogenesis, including the gene encoding the sexual commitment regulator AP2-G. Additionally, lipidomics of PNPLA1-deficient asexual blood stage parasites revealed overall increased levels of major phospholipids, including phosphatidylcholine (PC), which is a substrate of PLA2 . PC synthesis is known to be pivotal for erythrocytic replication, while the reduced availability of PC precursors drives the parasite into gametocytogenesis; we thus hypothesize that the higher PC levels due to PNPLA1-deficiency prevent the blood stage parasites from entering the sexual pathway.
Databáze: OpenAIRE
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