A Causative Relationship Exists Between Eosinophils and the Development of Allergic Pulmonary Pathologies in the Mouse
| Autor: | Stephania A. Cormier, Sergei I. Ochkur, Michael P. McGarry, Michael T. Borchers, Edie M. Hines, Hua Hao H. Shen, J. Paul Justice, K.R. O'Neill, Tracy Ansay, Jeffrey R. Crosby, Huiying Wang, Travis L. Biechelle, James J. Lee, Nancy A. Lee, Dana Colbert |
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| Rok vydání: | 2003 |
| Předmět: |
CD4-Positive T-Lymphocytes
Adoptive cell transfer Pathology medicine.medical_specialty Ovalbumin Immunology Mice Transgenic Inflammation Respiratory Mucosa Mice Intubation Intratracheal Respiratory Hypersensitivity Animals Immunology and Allergy Medicine Pulmonary pathology Pulmonary Eosinophilia Respiratory system Lung Aerosols Mice Knockout Eosinophil cationic protein medicine.diagnostic_test business.industry Allergens respiratory system Eosinophil medicine.disease Adoptive Transfer Eosinophils Mice Inbred C57BL Disease Models Animal Mucus Bronchoalveolar lavage medicine.anatomical_structure Bronchial Hyperreactivity Interleukin-5 medicine.symptom business |
| Zdroj: | The Journal of Immunology. 170:3296-3305 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.170.6.3296 |
| Popis: | Asthma and mouse models of allergic respiratory inflammation are invariably associated with a pulmonary eosinophilia; however, this association has remained correlative. In this report, a causative relationship between eosinophils and allergen-provoked pathologies was established using eosinophil adoptive transfer. Eosinophils were transferred directly into the lungs of either naive or OVA-treated IL-5−/− mice. This strategy resulted in a pulmonary eosinophilia equivalent to that observed in OVA-treated wild-type animals. A concomitant consequence of this eosinophil transfer was an increase in Th2 bronchoalveolar lavage cytokine levels and the restoration of intracellular epithelial mucus in OVA-treated IL-5−/− mice equivalent to OVA-treated wild-type levels. Moreover, the transfer also resulted in the development of airway hyperresponsiveness. These pulmonary changes did not occur when eosinophils were transferred into naive IL-5−/− mice, eliminating nonspecific consequences of the eosinophil transfer as a possible explanation. Significantly, administration of OVA-treated IL-5−/− mice with GK1.5 (anti-CD4) Abs abolished the increases in mucus accumulation and airway hyperresponsiveness following adoptive transfer of eosinophils. Thus, CD4+ T cell-mediated inflammatory signals as well as signals derived from eosinophils are each necessary, yet alone insufficient, for the development of allergic pulmonary pathology. These data support an expanded view of T cell and eosinophil activities and suggest that eosinophil effector functions impinge directly on lung function. |
| Databáze: | OpenAIRE |
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