In vivo and in vitro strategies to support caprine preantral follicle development after ovarian tissue vitrification
| Autor: | Johan Smitz, A.P.R. Rodrigues, R.R. Santos, Sheyla Farhayldes Souza Domingues, Marcelo Bertolini, Laritza Ferreira de Lima, Benner Geraldo Alves, Nathalie Jiatsa Donfack, K.A. Alves, R.M.P. Rocha, C.H. Lobo, Jamily Bezerra Bruno |
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| Přispěvatelé: | Follicle Biology, Pathology/molecular and cellular medicine, Clinical Biology |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Transplantation Heterotopic medicine.medical_treatment Reproductive technology Biology cryopreservation Cryopreservation Andrology Tissue Culture Techniques 03 medical and health sciences Follicle endocrinology 0302 clinical medicine Ovarian Follicle folliculogenesis Follicular phase medicine Animals genetics Molecular Biology heterotopic transplantation 030219 obstetrics & reproductive medicine Goats goat Antral follicle Vitrification Autotransplantation Transplantation 030104 developmental biology surgical procedures operative Female Animal Science and Zoology Folliculogenesis biotechnology reproductive medicine Developmental Biology |
| Popis: | The aim of the present study was to compare fresh and vitrified goat ovarian tissue after autotransplantation and in vitro culture. Adult goats were completely ovariectomised and each ovarian pair was sliced and distributed among six different treatment groups: fresh control, fresh transplant, fresh culture, vitrified control, vitrified transplant and vitrified culture. Follicular morphology, development, growth, density, revascularisation and hormone production were evaluated in all groups. Three antral follicles (two in the fresh transplant and one in the vitrified transplant groups) were observed on the surface of the graft 90 days after transplantation. The percentage of morphologically normal follicles was similar in the fresh control, fresh transplant and vitrified transplant groups. The percentage of developing (transition, primary and secondary) follicles was higher after in vitro culture of fresh or vitrified tissue. Transplantation resulted in a lower follicle density. Serum oestradiol concentrations remained constant during the entire transplantation period. In contrast, progesterone production decreased significantly. Expression of CD31 mRNA was lower in fresh culture. In conclusion, restoration of goat ovarian function can be successfully achieved following transplantation of both fresh and vitrified goat ovarian tissue. However, transplantation induced higher follicle loss than in vitro culture. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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