SERUM AND KNEE SYNOVIAL FLUID MATRIXMETALLOPROTEINASE-13, TUMOR NECROSIS FACTOR-ALPHA LEVELS IN PATIENTS WITH LATE STAGE OSTEOARTHRITIS
| Autor: | Kenan Ozler, Barış Yılmaz, Şafak Güngör, Erdem Aktaş, Çiğdem Atay, Murat Arikan |
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| Rok vydání: | 2015 |
| Předmět: |
Male
musculoskeletal diseases 0301 basic medicine medicine.medical_specialty WOMAC medicine.medical_treatment Arthritis Osteoarthritis Gastroenterology Serology 03 medical and health sciences 0302 clinical medicine lcsh:Orthopedic surgery Internal medicine Matrix Metalloproteinase 13 Synovial Fluid Humans Medicine Synovial fluid Serologic Tests Orthopedics and Sports Medicine Perioperative Period Arthroplasty Replacement Knee Aged Knee osteoarthritis matrix metalloproteinase TNF-alpha Tumor Necrosis Factor-alpha business.industry Venous blood General Medicine Middle Aged Osteoarthritis Knee medicine.disease Arthroplasty Surgery Matrix metalloproteinase lcsh:RD701-811 030104 developmental biology Original Article Female Tumor necrosis factor alpha business 030217 neurology & neurosurgery |
| Zdroj: | Acta Orthopaedica et Traumatologica Turcica Volume: 50, Issue: 3 Acta Orthopaedica et Traumatologica Turcica, Vol 50, Iss 6, Pp 670-673 (2016) |
| ISSN: | 1017-995X |
| DOI: | 10.3944/aott.2015.15.0115 |
| Popis: | Objective: To compare the levels of MMP-13 and TNF-α in late stage osteoarthritis, define their predominant pathways and investigate their correlation with McMaster Universities Arthritis Index scores. Patients and methods: A total of 42 patients (mean age 64 ± 8.8) with grade 3 and grade 4 knee osteoarthritis according to Kellegren- Lawrence criteria and who were scheduled for total knee arthroplasty were enrolled in the study. TNF-alpha and MMP-13 levels were measured preoperatively from venous blood samples and intraoperatively from knee synovial fluid via ELISA. Preoperative and 1 month postoperative knee functions were assessed by McMaster Universities Arthritis Index. Results: Grade 4 synovial fluid MMP-13 (4.76 ± 5.82) was elevated compared to grade 3 (3.95 ± 4.45) (p = 0.438), whereas grade 3 serum MMP-13 (1.128 ± 0.308) was found elevated compared to grade 4 (1.038 ± 0.204) (p = 0.430). Grade 4 serum TNF-α (0.253 ± 0.277) was elevated compared to grade 3 (0.206 ± 0.219) whereas grade 3 synovial fluid TNF-α (0.129 ± 0.052) was elevated compared to grade 4 (0.118 ± 0.014). Positive correlation was observed between synovial fluid MMP-13 levels and postoperative WOMAC scores. Mean serum TNF-α level (0.226 ± 0.246 pg/ml) was found higher compared to synovial level (0.124 ± 1.59), synovial MMP-13 level (4.31 ± 1.24) was found higher compared to serum level (1.089 ± 1.519). Conclusion: Despite the systemic increase in TNF-α levels concordant with osteoarthritis grade, MMP-13 levels are elevated via local manner with a significant correlation with WOMAC scores. Level of evidence: Level IV, Diagnostic study. Keywords: Matrix metalloproteinase, TNF-alpha, Knee osteoarthritis |
| Databáze: | OpenAIRE |
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