Aldo Keto Reductase 1B7 and Prostaglandin F2α Are Regulators of Adrenal Endocrine Functions
| Autor: | Jean-Christophe Pointud, Catherine Delarue, Hervé Lefebvre, François Coudoré, Antoine Martinez, Estelle Louiset, Sarah Lambert-Langlais, Isabelle Sahut-Barnola, Yoshihiro Urade, Fanny Volat, Bruno Ragazzon, M Manin, Pierre Val, Anne-Marie Lefrançois-Martinez |
|---|---|
| Přispěvatelé: | Unité de Nutrition Humaine (UNH), Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université-Institut National de la Recherche Agronomique (INRA), Génétique, Reproduction et Développement (GReD), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Clermont-Ferrand, Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Différenciation et communication neuronale et neuroendocrine (DC2N), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Génétique, Reproduction et Développement - Clermont Auvergne (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne (UCA)-Centre National de la Recherche Scientifique (CNRS), Neuroendocrinologie cellulaire et moléculaire |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Male
Chromaffin Cells Aldo-Keto Reductases lcsh:Medicine [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Dinoprost Diabetes and Endocrinology/Obesity Mice chemistry.chemical_compound 0302 clinical medicine Adrenal Glands Diabetes and Endocrinology/Endocrinology lcsh:Science ComputingMilieux_MISCELLANEOUS 0303 health sciences Multidisciplinary Adrenal cortex Adrenal gland respiratory system medicine.anatomical_structure lipids (amino acids peptides and proteins) hormones hormone substitutes and hormone antagonists Research Article endocrine system medicine.medical_specialty Prostaglandin Endocrine System Adrenocorticotropic hormone Biology Gene Expression Regulation Enzymologic 03 medical and health sciences Paracrine signalling Diabetes and Endocrinology/Adrenal Cortex Aldehyde Reductase Internal medicine medicine Animals Humans Rats Wistar Autocrine signalling Glucocorticoids 030304 developmental biology Aldo-keto reductase Binding Sites lcsh:R Rats Alcohol Oxidoreductases Endocrinology Glucocorticoid secretion chemistry Cyclooxygenase 2 lcsh:Q 030217 neurology & neurosurgery |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2009, 4 (10), pp.e7309. ⟨10.1371/journal.pone.0007309⟩ PLoS ONE, 2009, 4 (10), pp.e7309. ⟨10.1371/journal.pone.0007309⟩ PLoS ONE, Vol 4, Iss 10, p e7309 (2009) |
| ISSN: | 1932-6203 |
| Popis: | Prostaglandin F(2alpha) (PGF(2alpha)), represses ovarian steroidogenesis and initiates parturition in mammals but its impact on adrenal gland is unknown. Prostaglandins biosynthesis depends on the sequential action of upstream cyclooxygenases (COX) and terminal synthases but no PGF(2alpha) synthases (PGFS) were functionally identified in mammalian cells. In vitro, the most efficient mammalian PGFS belong to aldo-keto reductase 1B (AKR1B) family. The adrenal gland is a major site of AKR1B expression in both human (AKR1B1) and mouse (AKR1B3, AKR1B7). Thus, we examined the PGF(2alpha) biosynthetic pathway and its functional impact on both cortical and medullary zones. Both compartments produced PGF(2alpha) but expressed different biosynthetic isozymes. In chromaffin cells, PGF(2alpha) secretion appeared constitutive and correlated to continuous expression of COX1 and AKR1B3. In steroidogenic cells, PGF(2alpha) secretion was stimulated by adrenocorticotropic hormone (ACTH) and correlated to ACTH-responsiveness of both COX2 and AKR1B7/B1. The pivotal role of AKR1B7 in ACTH-induced PGF(2alpha) release and functional coupling with COX2 was demonstrated using over- and down-expression in cell lines. PGF(2alpha) receptor was only detected in chromaffin cells, making medulla the primary target of PGF(2alpha) action. By comparing PGF(2alpha)-responsiveness of isolated cells and whole adrenal cultures, we demonstrated that PGF(2alpha) repressed glucocorticoid secretion by an indirect mechanism involving a decrease in catecholamine release which in turn decreased adrenal steroidogenesis. PGF(2alpha) may be regarded as a negative autocrine/paracrine regulator within a novel intra-adrenal feedback loop. The coordinated cell-specific regulation of COX2 and AKR1B7 ensures the generation of this stress-induced corticostatic signal. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|