Live and inactivated vaccine regimens against caecal Salmonella Typhimurium colonisation in laying hens
| Autor: | Sue M. Sharpe, Julian Cox, Anthony Pavic, W. I. Muir, Peter J. Groves |
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| Rok vydání: | 2016 |
| Předmět: |
Salmonella typhimurium
0301 basic medicine Serotype Avian Salmonella Time Factors Salmonella Vaccines 040301 veterinary sciences 030106 microbiology Vaccines Attenuated medicine.disease_cause Microbiology 0403 veterinary science 03 medical and health sciences medicine Animals Cecum Poultry Diseases Salmonella Infections Animal Attenuated vaccine General Veterinary biology Aroa poultry Australia 04 agricultural and veterinary sciences General Medicine Salmonella vaccine vaccines biology.organism_classification Virology Vaccination Vaccines Inactivated Inactivated vaccine Female Flock layer chickens Chickens |
| Zdroj: | Australian Veterinary Journal |
| ISSN: | 1751-0813 0005-0423 |
| DOI: | 10.1111/avj.12490 |
| Popis: | Objective In Australia, Salmonella serovar Typhimurium (S. Typhimurium) is the predominant zoonotic serovar in humans and is frequently isolated from layer hens. Vaccination against this serovar has been previously shown to be effective in broilers and the aim of this current study was to assess and determine the best vaccination strategy (live or inactivated) to minimise caecal colonisation by S. Typhimurium. Methods A long-term experiment (56 weeks) was conducted on ISABROWN pullets using a commercial live aroA deleted mutant S. Typhimurium vaccine and an autogenous inactivated multivalent Salmonella vaccine (containing serovars Typhimurium, Infantis, Montevideo and Zanzibar). These vaccines were administered PO or by SC or IM injection, either alone or in combination. Pullets were vaccinated throughout rearing (to 18 weeks of age) and sequentially bled for antibody titre levels. The birds, vaccinated and controls, were challenged orally with a field isolate of S. Typhimurium at different ages, held for 21 days post-challenge, then euthanased and their caeca cultured for the presence of Salmonella. Results None of the oral live-vaccinated groups exhibited lasting protection. When administered twice, the inactivated vaccine gave significant protection at 17 weeks of age and the live vaccine given by SC injection given twice produced significant protection at 17, 25 and 34 weeks. Conclusions Vaccination regimens that included parenteral administration of live or inactivated vaccines and thus achieved positive serum antibody levels were able to provide protection against challenge. Hence, vaccination may play a useful role in a management strategy for Salmonella carriage in layer flocks. |
| Databáze: | OpenAIRE |
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