IgG3 and IgM Identified as Key to SARS-CoV-2 Neutralization in Convalescent Plasma Pools

Autor: Christina Kober, Sandro Manni, Svenja Wolff, Thomas Barnes, Shatanik Mukherjee, Thomas Vogel, Lea Hoenig, Peter Vogel, Aaron Hahn, Michaela Gerlach, Martin Vey, Eleonora Widmer, Björn Keiner, Patrick Schuetz, Nathan Roth, Uwe Kalina
Jazyk: angličtina
Rok vydání: 2022
Předmět:
RNA viruses
Viral Diseases
Coronaviruses
Physiology
Antibody Response
Antibodies
Viral
Biochemistry
Binding Analysis
Medical Conditions
Antigen Encapsulation
Immune Physiology
Chlorocebus aethiops
Enzyme-Linked Immunoassays
Drug Delivery System Preparation
Immune Response
Pathology and laboratory medicine
Multidisciplinary
Immune System Proteins
Pharmaceutics
Medical microbiology
Body Fluids
Blood
Infectious Diseases
Viruses
Medicine
SARS CoV 2
Pathogens
Anatomy
Research Article
SARS coronavirus
Science
Immunology
Enzyme-Linked Immunosorbent Assay
Research and Analysis Methods
Microbiology
Antibodies
Blood Plasma
Animals
Humans
Immunoassays
Vero Cells
Chemical Characterization
Medicine and health sciences
Biology and life sciences
Pharmaceutical Processing Technology
SARS-CoV-2
Organisms
Viral pathogens
Proteins
COVID-19
Covid 19
Convalescence
Antibodies
Neutralizing
Microbial pathogens
Immunoglobulin M
Immunoglobulin G
Immunologic Techniques
Zdroj: PLoS ONE
PLoS ONE, Vol 17, Iss 1, p e0262162 (2022)
PLoS ONE, Vol 17, Iss 1 (2022)
ISSN: 1932-6203
Popis: Analysis of convalescent plasma derived from individuals has shown that IgG3 has the most important role in binding to SARS-CoV-2 antigens; however, this has not yet been confirmed in large studies, and the link between binding and neutralization has not been confirmed. By analyzing plasma pools consisting of 247–567 individual convalescent donors, we demonstrated the binding of IgG3 and IgM to Spike-1 protein and the receptor-binding domain correlates strongly with viral neutralization in vitro. Furthermore, despite accounting for only approximately 12% of total immunoglobulin mass, collectively IgG3 and IgM account for approximately 80% of the total neutralization. This may have important implications for the development of potent therapies for COVID-19, as it indicates that hyperimmune globulins or convalescent plasma donations with high IgG3 concentrations may be a highly efficacious therapy.
Databáze: OpenAIRE
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