The minimal gene set member msrA, encoding peptide methionine sulfoxide reductase, is a virulence determinant of the plant pathogen Erwinia chrysanthemi
| Autor: | Frédérique Van Gijsegem, Mohammed El Hassouni, Jean Pierre Chambost, Frédéric Barras, Dominique Expert |
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| Rok vydání: | 1999 |
| Předmět: |
Mutant
Molecular Sequence Data Pectobacterium chrysanthemi Virulence Biology medicine.disease_cause Genes Plant Polymerase Chain Reaction Microbiology medicine Escherichia coli Cloning Molecular Pathogen Multidisciplinary Base Sequence Structural gene Dickeya chrysanthemi Plants Biological Sciences Recombinant Proteins Oxidative Stress Biochemistry Methionine Sulfoxide Reductases Methionine sulfoxide reductase Oxidoreductases MSRA |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 96(3) |
| ISSN: | 0027-8424 |
| Popis: | Peptide methionine sulfoxide reductase (MsrA), which repairs oxidized proteins, is present in most living organisms, and the cognate structural gene belongs to the so-called minimum gene set [Mushegian, A. R. & Koonin, E. V., (1996) Proc. Natl. Acad. Sci. USA 93, 10268–10273]. In this work, we report that MsrA is required for full virulence of the plant pathogen Erwinia chrysanthemi . The following differences were observed between the wild-type and a MsrA − mutant: ( i ) the MsrA − mutant was more sensitive to oxidative stress; ( ii ) the MsrA − mutant was less motile on solid surface; ( iii ) the MsrA − mutant exhibited reduced virulence on chicory leaves; and ( iv ) no systemic invasion was observed when the MsrA − mutant was inoculated into whole Saintpaulia ionantha plants. These results suggest that plants respond to virulent pathogens by producing active oxygen species, and that enzymes repairing oxidative damage allow virulent pathogens to survive the host environment, thereby supporting the theory that active oxygen species play a key role in plant defense. |
| Databáze: | OpenAIRE |
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