Matrix-specified differentiation of human decidua parietalis placental stem cells
| Autor: | Indumathi Sridharan, Zuzana Strakova, Taeyoung Kim, Rong Wang |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Cellular differentiation
Decidua Parietalis Biophysics Cell Enlargement Biology Placenta cord banking Microscopy Atomic Force Biochemistry Article Collagen Type I Extracellular matrix Directed differentiation Tissue engineering Decidua Humans Cell Lineage Molecular Biology Cells Cultured Embryonic Stem Cells Neurons Nanotubes Carbon Integrin beta1 Cell Differentiation Cell Biology Embryonic stem cell Extracellular Matrix Cell biology Immunology Female Stem cell |
| Zdroj: | Biochemical and Biophysical Research Communications. 437:489-495 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2013.07.002 |
| Popis: | To create suitable biological scaffolds for tissue engineering and cell therapeutics, it is essential to understand the matrix-mediated specification of stem cell differentiation. To this end, we studied the effect of collagen type I on stem cell lineage specification. We altered the properties of collagen type I by incorporating carbon nanotubes (CNT). The collagen-CNT composite material was stiffer with thicker fibers and longer D-period. Human decidua parietalis stem cells (hdpPSC) were found to differentiate exclusively and rapidly towards neural cells on the collagen-CNT matrix. We attribute this accelerated neural differentiation to the enhanced structural and mechanical properties of collagen-CNT material. Strikingly, the collagen-CNT matrix, unlike collagen, imposes the neural fate by an alternate mechanism that may be independent of beta-1 integrin and beta-catenin. The study demonstrates the sensitivity of stem cells to subtle changes in the matrix and the utilization of a novel biocomposite material for efficient and directed differentiation of stem cells. |
| Databáze: | OpenAIRE |
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