Altered expression of microRNAs targeting Dkk-1 in peripheral blood mononuclear cells of patients with ankylosing spondylitis
| Autor: | Guoxiang Song, Zhongchao Fu, Z. Yin, Lijun Zhang, Jinxian Huang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
microrna
Immunology lcsh:Medicine Peripheral blood mononuclear cell Pathogenesis 03 medical and health sciences wnt pathway 0302 clinical medicine ankylosing spondylitis Immunology and Allergy Medicine BASDAI dkk-1 Ankylosing spondylitis medicine.diagnostic_test business.industry lcsh:R Wnt signaling pathway medicine.disease DKK1 Erythrocyte sedimentation rate Clinical Immunology business BASFI 030215 immunology |
| Zdroj: | Central European Journal of Immunology, Vol 44, Iss 1, Pp 59-64 (2019) Central-European Journal of Immunology |
| ISSN: | 1644-4124 1426-3912 |
| Popis: | Introduction Ankylosing spondylitis (AS) is a chronic inflammatory disease characterised by new bone formation, and Dickkopf homologue 1 (Dkk-1) may contribute to the ankylosis of the sacroiliac joint as a main regulator of the Wingless (Wnt) pathway. Increasing evidence shows that microRNAs targeting Dkk-1 might play a critical role in the pathogenesis of rheumatic diseases. We aim to investigate alterations in expression of miRNAs targeting Dkk-1 in AS patients in this study. Material and methods The peripheral blood mononuclear cells (PBMCs) of 20 AS patients and 20 normal controls were collected in our study. Three miRNAs targeting DKK1 including miR-29a, miR-335, and miR-363 were selected and quantitative real-time PCR was used to identify the expression of the three miRNAs in these samples. Correlation analysis was conducted between altered miRNA expression and erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), Bath Ankylosing Spondylitis Disease Activity Index (BASDAI), Bath Ankylosing Spondylitis Function Index (BASFI), and mSASSS (modified Stoke Ankylosing Spondylitis Spinal Score). Results The expression of miR-29a was significantly higher in AS patients than in healthy controls (p < 0.01), while no significance was observed in the expression of miR-335 and miR-363 between AS patients and healthy controls (p > 0.05). No correlation was observed between miR-29a and ESR, CRP, BASDAI, and BASFI (p > 0.05). The elevated miR-29a expression was correlated with disease duration and mSASSS (p < 0.05). Conclusions MiR-29a might be a useful marker in AS new bone formation and contributes to the regulation of Dkk-1 in Wnt signalling. |
| Databáze: | OpenAIRE |
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