Effects of salbutamol on exhaled breath condensate biomarkers in acute lung injury: prospective analysis
| Autor: | Oriol Roca, Susana Gómez-Ollés, Xavier Muñoz, María Jesús Cruz, Joan R. Masclans, Mark J.D. Griffiths |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Male
Lung injury Dinoprost Critical Care and Intensive Care Medicine Leukotriene B4 Severity of Illness Index Prospective analysis Adrenergic beta-Agonists Humans Medicine Albuterol Exhaled breath condensate Metered Dose Inhalers Prospective Studies Prospective cohort study Nitrites Aged Respiratory Distress Syndrome Nitrates Lung Pulmonary Gas Exchange business.industry Research Exhalation Hydrogen-Ion Concentration Middle Aged respiratory system Respiration Artificial respiratory tract diseases medicine.anatomical_structure Breath Tests Anesthesia Salbutamol Female business Biomarkers medicine.drug |
| Zdroj: | Critical Care |
| Popis: | Introduction The benefits of β-adrenergic stimulation have been described in acute lung injury (ALI), but there is still no evidence of its anti-inflammatory effect in these patients. Biomarkers in exhaled breath condensate (EBC) were used to study the effects of salbutamol on lung inflammation in mechanically ventilated patients with ALI. Methods EBC was collected before and 30 minutes after administration of inhaled salbutamol (800 μg). The following parameters were measured in the samples: volume obtained, conductivity, pH after helium deaeration, and concentration of nitrites, nitrates and 8-isoprostane. The leukotriene B4 concentration was measured after sample lyophilization and reconstitution. Results are expressed as the median (interquartile range). Results EBC was obtained from six ALI patients, with a median age of 56 (46 to 76) years. At the time of EBC collection, the Lung Injury Score was 3 (2.3 to 3.1) and the PaO2/FIO2 ratio was 133 (96 to 211) mmHg. A significant increase in deaerated EBC pH was observed after salbutamol administration (7.66 (7.58 to 7.75) versus 7.83 (7.67 to 7.91), P = 0.028). Trends toward decreased nitrosative species (18.81 (13.33 to 49.44) μM versus 21.21 (8.07 to 29.83) μM, P = 0.173) and decreased 8-isoprostane concentration (11.64 (7.17 to 17.13) pg/ml versus 6.55 (4.03 to 9.99) pg/ml, P = 0.068) were detected. No changes in leukotriene B4 concentration were found (1.58 (0.47 to 3.57) pg/ml versus 2.06 (1.01 to 3.01) pg/ml, P = 0.753). Conclusion EBC analysis is a noninvasive technique that can be used to monitor ventilated patients. In EBC from a small cohort of patients with ALI, inhaled salbutamol significantly decreased airspace acidosis, a marker of inflammation, and was associated with a trend toward decreased markers of nitrosative and oxidative stress. |
| Databáze: | OpenAIRE |
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