Intraocular BDNF promotes ectopic branching, alters motility and stimulates abnormal collaterals in regenerating optic fibers
| Autor: | Amy J. Dawson, Jill Miotke, Ronald L. Meyer |
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| Rok vydání: | 2015 |
| Předmět: |
1.1 Normal biological development and functioning
Central nervous system Motility Injury Biology Regenerative Medicine Axon Article Retina Underpinning research Goldfish Neural Pathways medicine Psychology Animals Molecular Biology Brain-derived neurotrophic factor Tectum Mesencephali Neurology & Neurosurgery Brain-Derived Neurotrophic Factor General Neuroscience Neurosciences Trophic factor Optic Nerve Axons Nerve Regeneration medicine.anatomical_structure nervous system Optic Nerve Injuries In vivo imaging Neurological Optic nerve Cognitive Sciences Neurology (clinical) Visual system Tectum Neuroscience Developmental Biology |
| Zdroj: | Brain Research. 1613:13-26 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/j.brainres.2015.03.045 |
| Popis: | A great deal of effort has been invested in using trophic factors and other bioactive molecules to promote cell survival and axonal regeneration in the adult central nervous system. Far less attention has been paid to investigating potential effects that trophic factors may have that might interfere with recovery. In the visual system, BDNF has been previously reported to prevent regeneration. To test if BDNF is inherently incompatible with regeneration, BDNF was given intraocularly during optic nerve regeneration in the adult goldfish. In vivo imaging and anatomical analysis of selectively labeled axons were used as a sensitive assay for effects on regeneration within the tectum. BDNF had no detectable inhibitory effect on the ability of axons to regenerate. Normal numbers of axons regenerated into the tectum, exhibited dynamic growth and retractions similar to controls, and were able to navigate to their correct target zone in the tectum. However, BDNF was found to have additional effects that adversely affected the quality of regeneration. It promoted premature branching at ectopic locations, diminished the growth rate of axons through the tectum, and resulted in the formation of ectopic collaterals. Thus, although BDNF has robust effects on axonal behavior, it is, nevertheless, compatible with axonal regeneration, axon navigation and the formation of terminal arbors. |
| Databáze: | OpenAIRE |
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