Towards Novel Anti-tumor Strategies for Hepatic Cancer: ɛ-Viniferin in Combination with Vincristine Displays Pharmacodynamic Synergy at Lower Doses in HepG2 Cells
| Autor: | Zerrin İncesu, Arzu Işcan, Mesut Şen, H. Mehtap Kutlu, Nur İpek Önder, Gülşen Akalın, Filiz Özdemir |
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| Přispěvatelé: | Anadolu Üniversitesi, Eczacılık Fakültesi, Biyokimya Anabilim Dalı, Akalın Çiftçi, Gülsen, İşcan, Arzu, Kutlu, Hatice Mehtap, Seller, Zerrin |
| Rok vydání: | 2014 |
| Předmět: |
Vincristine
Carcinoma Hepatocellular Cell Survival Apoptosis DNA Fragmentation Pharmacology Biology Biochemistry Inhibitory Concentration 50 chemistry.chemical_compound Stilbenes Genetics medicine Humans MTT assay Viability assay Propidium iodide Cell Shape Molecular Biology Benzofurans Dose-Response Relationship Drug Liver Neoplasms Drug Synergism Original Articles Hep G2 Cells Antineoplastic Agents Phytogenic chemistry Agarose gel electrophoresis Molecular Medicine DNA fragmentation Drug Screening Assays Antitumor Growth inhibition Biotechnology medicine.drug |
| Zdroj: | OMICS: A Journal of Integrative Biology. 18:324-334 |
| ISSN: | 1557-8100 1536-2310 |
| DOI: | 10.1089/omi.2013.0045 |
| Popis: | WOS: 000335737800005 PubMed ID: 24341688 Hepatocellular carcinoma is the fifth most common cancer and the third leading cause of cancer-related deaths worldwide. The efficacy of novel combination treatments are increasingly evaluated with use of integrative biology research and development (R&D) strategies and methodological triangulation. We investigated the anti-tumor effect of -viniferin alone, and the putative synergy of -viniferin with vincristine on the growth of HepG2 cells in vitro. Growth inhibition and apoptosis induction were determined by MTT assay and annexin V/propidium iodide (PI), respectively. Morphological changes and DNA fragmentation were investigated under electron microscopy and by agarose gel electrophoresis, respectively. The results collectively showed that treating cells with -viniferin and vincristine significantly inhibited cell viability at lower doses as compared to each agent applied alone. IC50 values for -viniferin and vincristine were determined as 98.3 and 52.5M at 24h, respectively. IC50 value of -viniferin in combination with vincristine was 15.8+11.25M (mean/SD) at 24h. The viability of cells treated with 17.9M vincristine alone for 24h was 79.62%; it reduced to 26.53% when 25M -viniferin was added in combination with vincristine (p Anadolu University [090306] This work was funded by a grant from the Anadolu University (Project No. 090306). Authors wish to thank BIBAM for technical support. |
| Databáze: | OpenAIRE |
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