Chronicl-DOPA treatment differentially regulates gene expression of glutamate decarboxylase, preproenkephalin and preprotachykinin in the striatum of 6-hydroxydopamine-lesioned rat
| Autor: | C.D. Marsden, Bai-Yun Zeng, J. Jolkkonen, Peter Jenner |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Preprotachykinin
medicine.medical_specialty Molecular Sequence Data Glutamate decarboxylase Substance P In situ hybridization Striatum Biology Gene Expression Regulation Enzymologic Levodopa chemistry.chemical_compound Tachykinins Internal medicine Gene expression medicine Animals RNA Messenger Protein Precursors Rats Wistar Oxidopamine In Situ Hybridization Messenger RNA Base Sequence Behavior Animal Glutamate decarboxylase activity Glutamate Decarboxylase General Neuroscience Carbidopa Enkephalins Rats Neostriatum Endocrinology chemistry Biochemistry Female Oligonucleotide Probes |
| Zdroj: | Neuroscience. 66:19-28 |
| ISSN: | 0306-4522 |
| DOI: | 10.1016/0306-4522(94)00574-o |
| Popis: | The effect of a unilateral 6-hydroxydopamine lesion of the medial forebrain bundle in rats and subsequent L-DOPA treatment for eight weeks on preproenkephalin, preprotachykinin and glutamate decarboxylase (M(r) 67,000) gene expression in the striatum was investigated by in situ hybridization. A 6-hydroxydopamine lesion of the medial forebrain bundle markedly increased the level of preproenkephalin messenger RNA (+66%) and modestly elevated the level of glutamate decarboxylase (M(r) 67,000) messenger RNA (+36%) in the denervated striatum, but caused a decrease in the level of preprotachykinin messenger RNA (-54%) relative to the intact striatum and to sham-lesioned control animals. Treatment with L-DOPA (200 mg/kg/24 h) for eight weeks reduced but did not abolish the 6-hydroxydopamine lesion-induced elevation of preproenkephalin messenger RNA and slightly reduced the elevation of glutamate decarboxylase (M(r) 67,000) messenger RNA in denervated striatum relative to intact side and control groups. However, L-DOPA treatment almost completely reversed the decrease in preprotachykinin messenger RNA caused by 6-hydroxydopamine lesioning when compared to intact side and control groups. The effect of L-DOPA on the gene expression of preproenkephalin and glutamate decarboxylase (M(r) 67,000) differs from the increase in striatal enkephalin content and glutamate decarboxylase activity previously found following L-DOPA treatment. In contrast, L-DOPA reversed the changes in preprotachykinin messenger RNA, reflecting previously reported increases in substance P content. The findings provide new evidence that chronic L-DOPA treatment differentially affects direct striatonigral and indirect striatopallidal pathways at the molecular level. |
| Databáze: | OpenAIRE |
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