Two hybrid histidine kinases, TcsB and the phytochrome FphA, are involved in temperature sensing in Aspergillus nidulans
| Autor: | Kai Leister, Zhenzhong Yu, Arin Ali, Tilman Lamparter, Norbert Krauß, Reinhard Fischer, Christian Streng, Olumuyiwa Igbalajobi |
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| Rok vydání: | 2019 |
| Předmět: |
Histidine Kinase
Light Reversion Microbiology Aspergillus nidulans Fungal Proteins 03 medical and health sciences chemistry.chemical_compound Thermosensing Molecular Biology Histidine 030304 developmental biology 0303 health sciences Biliverdin biology Phytochrome 030306 microbiology Kinase Histidine kinase Temperature Membrane Proteins biology.organism_classification chemistry Biophysics Mitogen-Activated Protein Kinases Protein Kinases Function (biology) |
| Zdroj: | Molecular microbiologyReferences. 112(6) |
| ISSN: | 1365-2958 |
| Popis: | The adaptation of microorganisms to different temperatures is an advantage in habitats with steadily changing conditions and raises the question about temperature sensing. Here we show that in the filamentous fungus Aspergillus nidulans, the hybrid histidine kinase TcsB and phytochrome are involved in temperature-induced gene transcription. Temperature-activated phytochrome fed the signal into the HOG MAP kinase pathway. There is evidence that the photoreceptor phytochrome fulfills a temperature sensory role in plants and bacteria. The effects in plants are based on dark reversion from the active form of phytochrome, Pfr, to the inactive form, Pr. Elevated temperature leads to higher dark reversion rates, and hence, temperature sensing depends on light. In A. nidulans and in Alternaria alternata, the temperature response was light-independent. In order to understand the primary temperature response of phytochrome, we performed spectral analyses of recombinant FphA from both fungi. Spectral properties after heat stress resembled the spectrum of free biliverdin, suggesting conformational changes and a softening of the binding pocket of phytochrome, possibly mimicking photoactivation. We propose a novel function for fungal phytochrome as temperature sensor. |
| Databáze: | OpenAIRE |
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