Organ-specific regulation of pro-inflammatory molecules in heart, lung, and kidney following brain death
| Autor: | Evgenij V. Potapov, Larry O. Thompson, George P. Noon, David L. Joyce, Matthias Loebe, Keith A. Youker, Robert E. Southard, C. A. Skrabal |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine Brain Death Pathology medicine.medical_specialty Swine medicine.medical_treatment Priming (immunology) Inflammation Kidney Internal medicine Organ specific Inflammatory molecules Animals Medicine RNA Messenger Lung Messenger RNA Interleukin-6 Tumor Necrosis Factor-alpha business.industry Cell adhesion molecule Gene Expression Profiling Myocardium Intercellular Adhesion Molecule-1 Receptors Interleukin-6 Cytokine medicine.anatomical_structure Organ Specificity Cardiology Cytokines Female Surgery Tumor necrosis factor alpha medicine.symptom Cardiology and Cardiovascular Medicine business Interleukin-1 |
| Zdroj: | Journal of Surgical Research. 123:118-125 |
| ISSN: | 0022-4804 |
| DOI: | 10.1016/j.jss.2004.07.245 |
| Popis: | Nonspecific inflammatory events following brain death may increase the intensity of the immunological host response. The present study investigated the course of pro-inflammatory molecules in heart, lung, kidney, and plasma after brain death induction.Brain death was induced in five pigs by inflation of an intracranial Foley catheter and five pigs were sham-operated as controls. Each experiment was terminated 6 h after brain death/sham operation and the organs were harvested. We measured the mRNA and protein levels for TNF-alpha, IL-1beta, and IL-6 in heart, lung, kidney, and plasma. Additionally, the mRNA expression for IL-6R, ICAM-1, MCP-1, and TGF-beta was determined in each organ.After 6 h, the plasma cytokine levels were higher in the brain-dead animals than in the sham-operated. In heart, lung, and kidney there was an increase in IL-6 and IL-1beta following brain death, while TNF-alpha was up-regulated in lung only (P0.05). MCP-1 and TGF-beta were significantly higher in heart and lung and IL-6R increased in heart after brain death (P0.05).Brain death was associated with non-uniform cytokine expression patterns in the investigated organs. These expression patterns may cause variable pro-inflammatory priming resulting in different degrees of damage and explain the organ-specific variation in outcomes after transplantations. |
| Databáze: | OpenAIRE |
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