DNA-induced increase in the .alpha.-helical content of C/EBP and GCN4
| Autor: | Jon Shuman, Karyn T. O'Neil, Christophe Ampe, William F. DeGrado |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Conformational change
Leucine zipper Saccharomyces cerevisiae Proteins Protein Conformation Molecular Sequence Data Biology Biochemistry DNA-binding protein Fungal Proteins Structure-Activity Relationship chemistry.chemical_compound Recognition sequence Structural motif Leucine Zippers Base Sequence Sulfates Circular Dichroism Nuclear Proteins bZIP domain DNA DNA-Binding Proteins chemistry CCAAT-Enhancer-Binding Proteins Biophysics Protein Kinases Transcription Factors Binding domain |
| Zdroj: | Biochemistry. 30:9030-9034 |
| ISSN: | 1520-4995 0006-2960 |
| DOI: | 10.1021/bi00101a017 |
| Popis: | Leucine zipper proteins comprise a recently identified class of DNA binding proteins that contain a bipartite structural motif consisting of a "leucine zipper" dimerization domain and a segment rich in basic residues responsible for DNA interaction. Protein fragments encompassing the zipper plus basic region domains (bZip) have previously been used to determine the conformational and dynamic properties of this motif. In the absence of DNA, the coiled-coil portion is alpha-helical and dimeric, whereas the basic region is flexible and partially disordered. Addition of DNA containing a specific recognition sequence induces a fully helical conformation in the basic regions of these fragments. However, the question remained whether the same conformational change would be observed in native bZip proteins where the basic regions might be stabilized in an alpha-helical conformation even in the absence of DNA, through interactions with portions of the protein not included in the bZip motif. We have now examined the DNA-induced conformational transition for an intact bZip protein, GCN4, and for the bZip fragment of C/EBP with two enhancers that are differentially symmetric. Our results are consistent with the induced helical fork model wherein the basic regions are largely flexible in the absence of DNA and become fully helical in the presence of the specific DNA recognition sequence. |
| Databáze: | OpenAIRE |
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