Differential impact of oleate, palmitate, and adipokines on expression of NF-κB target genes in human vascular smooth muscle cells
| Autor: | Daniela Lamers, Henrike Sell, A Horrighs, Juergen Eckel, Raphaela Schlich, A Cramer |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Vasculitis
medicine.medical_specialty Vascular smooth muscle Myocytes Smooth Muscle Palmitic Acid Gene Expression Adipose tissue Adipokine Inflammation Biology Biochemistry Muscle Smooth Vascular Palmitic acid chemistry.chemical_compound Endocrinology Adipokines Cell Movement Internal medicine Adipocytes medicine Humans Myocyte Obesity Chemokine CCL5 Molecular Biology Transcription factor Cells Cultured Cell Proliferation Interleukin-6 Tumor Necrosis Factor-alpha NF-kappa B NF-κB Middle Aged Atherosclerosis Activins Gene Expression Regulation chemistry Culture Media Conditioned Female Matrix Metalloproteinase 1 medicine.symptom Apoptosis Regulatory Proteins Reactive Oxygen Species Angiopoietins Oleic Acid |
| Zdroj: | Molecular and Cellular Endocrinology. 362:194-201 |
| ISSN: | 0303-7207 |
| DOI: | 10.1016/j.mce.2012.06.010 |
| Popis: | It is widely accepted that obesity is a major risk factor for the development of atherosclerosis. In this context, adipose tissue produces a variety of adipokines and releases free fatty acids, contributing to a chronic-low grade inflammation state implicated in vascular complications. In this study, we investigated the role of adipokines, oleic acid (OA), palmitic acid (PA), and the combinations on activation of NF-κB target genes in human vascular smooth muscle cells (SMC) to assess the hypothesis of synergistic interactions between these molecules. Adipocyte-conditioned medium (CM), generated from human adipocytes, in combination with low concentrations of OA, but not PA, induces SMC proliferation and activation of the transcription factor NF-κB in a synergistic way. Combined treatment of CM and OA further regulates a set of downstream NF-κB target genes including angiopoietin-1, activin A, and MMP-1, all critically involved in SMC dysfunction. This suggests that the lipotoxic potential of fatty acids is substantially enhanced by the presence of adipocyte-derived factors. |
| Databáze: | OpenAIRE |
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