Bioinspired Dual-Enzyme Colloidosome Reactors for High-Performance Biphasic Catalysis
Autor: | Lei Wang, Wang Bingdi, Zhi-Da Wang, Zhenning Liu, Shenghui Jin, Liang Song |
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Rok vydání: | 2018 |
Předmět: |
Materials science
biology Aqueous two-phase system Ethyl acetate Ether 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology biology.organism_classification 01 natural sciences 0104 chemical sciences Catalysis chemistry.chemical_compound Adsorption chemistry Chemical engineering Yield (chemistry) General Materials Science Candida antarctica Microreactor 0210 nano-technology |
Zdroj: | ACS Applied Materials & Interfaces. 10:41504-41511 |
ISSN: | 1944-8252 1944-8244 |
Popis: | In this paper, a novel method for the construction of colloidosomes as a microreactor for dual-enzyme cascade biphasic reaction has been reported. A lipase–glucose oxidase (GOx) enzyme pair is employed in this system. A water-soluble enzyme GOx is compartmentalized inside the colloidosomes. A hydrophobic environment-favored enzyme Candida Antarctica lipase B (CalB) is adsorbed on the outer surfaces of the colloidosomes. The catalysis system is set up by introducing these dual-enzyme-immobilized microcapsules into acetic ether. H2O2 is produced in the aqueous phase by the doped GOx, and then H2O2 diffused out of the microcapsules is utilized by CalB to catalyze the oxidation of ethyl acetate. Finally, the formed peracids oxidized N-heteroaromatic in situ. Furthermore, no obvious yield decline is observed in four reaction cycles. Thus, our work provides a new strategy for the design of high-performance biomimicking reactors for multiple enzyme cascade reactions and further expands the potential application ... |
Databáze: | OpenAIRE |
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