Seselin from Plumbago zeylanica inhibits phytohemagglutinin (PHA)-stimulated cell proliferation in human peripheral blood mononuclear cells
| Autor: | Kai-An Chuang, Ming-Hsi Wu, Shi Chuan Chang, Yi-Chun Chen, Wei-Jern Tsai, Lie Chwen Lin, Yuh-Chi Kuo |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Adult
Male Plumbago zeylanica Cell Survival Biology Peripheral blood mononuclear cell Inhibitory Concentration 50 Interferon-gamma Young Adult Plumbaginaceae Coumarins Drug Discovery Gene expression Humans Immunologic Factors Viability assay Phytohemagglutinins Cytotoxicity Cell Proliferation Pharmacology Dose-Response Relationship Drug Plant Extracts Cell growth Cell Cycle Cell cycle biology.organism_classification Molecular biology Dose–response relationship Gene Expression Regulation Immunology Leukocytes Mononuclear Interleukin-2 |
| Zdroj: | Journal of Ethnopharmacology. 119:67-73 |
| ISSN: | 0378-8741 |
| Popis: | Effects of seselin (C(14)H(12)O(3); MW 228) identified from Plumbago zeylanica on phytohemagglutinin (PHA)-stimulated cell proliferation were studied in human peripheral blood mononuclear cells (PBMC). The data demonstrated that seselin inhibited PBMC proliferation-activated with PHA with an IC(50) of 53.87+/-0.74 microM. Cell viability test indicated that inhibitory effects of seselin on PBMC proliferation were not through direct cytotoxicity. The action mechanisms of seselin may involve the regulation of cell cycle progression, interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) production in PBMC. Since cell cycle analysis indicated that seselin arrested the cell cycle progression of activated PBMC from the G(1) transition to the S phase. Seselin suppressed IL-2 and IFN-gamma production in a concentration-dependent manner. Furthermore, seselin significantly decreased the IL-2 and IFN-gamma gene expression in PHA-activated PBMC. Therefore, results elucidated for the first time that seselin is likely an immunomodulatory agent for PBMC. |
| Databáze: | OpenAIRE |
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