Carbon fixation and rhodopsin systems in microbial mats from hypersaline lakes Brava and Tebenquiche, Salar de Atacama, Chile

Autor: Manuel Contreras, Maria Eugenia Farias, Dario Ezequiel Elias, María Cecilia Rasuk, Daniel Kurth
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Geologic Sediments
Salinity
Physiology
Marine and Aquatic Sciences
Plant Science
carbone fixation
Biochemistry
Plant Tropisms
purl.org/becyt/ford/1 [https]
RNA
Ribosomal
16S
Environmental DNA
Microbial mat
Photosynthesis
Chile
Phylogeny
Data Management
Plant Growth and Development
Multidisciplinary
biology
Ecology
Plant Biochemistry
Microbiota
Chemical Reactions
Genomics
Biodiversity
Chemistry
Plant Physiology
Physical Sciences
Medicine
Proteobacteria
Research Article
Freshwater Environments
Computer and Information Sciences
Multiple Alignment Calculation
Rhodopsin
Firmicutes
Science
Cyanobacteria
Research and Analysis Methods
Tropism
Carbon Cycle
Oxidation
Computational Techniques
Extremophile
Genetics
metagenomic
Phototropism
purl.org/becyt/ford/1.6 [https]
Taxonomy
Bacteria
Whole Genome Sequencing
Phylum
Bacteroidetes
Ecology and Environmental Sciences
Organisms
Biology and Life Sciences
Aquatic Environments
Bodies of Water
biology.organism_classification
salt pads microbiology
Anoxygenic photosynthesis
Split-Decomposition Method
Lakes
Metagenomics
Earth Sciences
Organism Development
Developmental Biology
Zdroj: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
PLoS ONE
PLoS ONE, Vol 16, Iss 2, p e0246656 (2021)
Popis: In this work, molecular diversity of two hypersaline microbial mats was compared by Whole Genome Shotgun (WGS) sequencing of environmental DNA from the mats. Brava and Tebenquiche are lakes in the Salar de Atacama, Chile, where microbial communities are growing in extreme conditions, including high salinity, high solar irradiance, and high levels of toxic metals and metaloids. Evaporation creates hypersaline conditions in these lakes and mineral precipitation is a characteristic geomicrobiological feature of these benthic ecosystems. The mat from Brava was more rich and diverse, with a higher number of different taxa and with species more evenly distributed. At the phylum level, Proteobacteria, Cyanobacteria, Chloroflexi, Bacteroidetes and Firmicutes were the most abundant, including ~75% of total sequences. At the genus level, the most abundant sequences were affilitated to anoxygenic phototropic and cyanobacterial genera. In Tebenquiche mats, Proteobacteria and Bacteroidetes covered ~70% of the sequences, and 13% of the sequences were affiliated to Salinibacter genus, thus addressing the lower diversity. Regardless of the differences at the taxonomic level, functionally the two mats were similar. Thus, similar roles could be fulfilled by different organisms. Carbon fixation through the Wood-Ljungdahl pathway was well represented in these datasets, and also in other mats from Andean lakes. In spite of presenting less taxonomic diversity, Tebenquiche mats showed increased abundance and variety of rhodopsin genes. Comparison with other metagenomes allowed identifying xantorhodopsins as hallmark genes not only from Brava and Tebenquiche mats, but also for other mats developing at high altitudes in similar environmental conditions. Fil: Kurth, Daniel German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Elias, Dario Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno". CEMIC-CONICET; Argentina Fil: Rasuk, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Contreras, Emanuel. Centro de Ecología Aplicada (CEA); Chile Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Databáze: OpenAIRE
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