Regulation of heme oxygenase 1 expression by miR-27b with stem cell therapy for liver regeneration in rats
| Autor: | Chih-Chi Wang, Yen-Ying Ma, Chia-Yun Lai, Wei-Teng Weng, Chih-Che Lin, Toshiaki Nakano, K. D. Chen, Yu Fan Cheng, Kuang-Tzu Huang, Shigeru Goto, Li-Wen Hsu, King-Wah Chiu, Yur-Ren Kuo, Chao-Long Chen |
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| Rok vydání: | 2013 |
| Předmět: |
Liver Cirrhosis
Male Pathology medicine.medical_specialty animal structures Time Factors medicine.medical_treatment Inflammation Biology Mesenchymal Stem Cell Transplantation Transfection Gene Expression Regulation Enzymologic Hepatitis Cell therapy medicine Animals Hepatectomy Enzyme Inhibitors Cells Cultured Cell Proliferation Transplantation Regeneration (biology) Mesenchymal stem cell Mesenchymal Stem Cells Stem-cell therapy Liver regeneration Liver Regeneration Heme oxygenase MicroRNAs Adipose Tissue Liver Rats Inbred Lew Heme Oxygenase (Decyclizing) Models Animal Cancer research Surgery Female RNA Interference medicine.symptom Myofibroblast |
| Zdroj: | Transplantation proceedings. 46(4) |
| ISSN: | 1873-2623 |
| Popis: | Adipose-derived mesenchymal stem cells (ASCs) have been considered to be attractive and readily available adult mesenchymal stem cells (MSCs) and are becoming increasingly popular for use in regenerating cell therapy. However, recent evidence attributed a fibrotic potential to MSCs which differentiated into myofibroblasts with highly increased α-smooth muscle actin (α-SMA) expression while transplanted into an injured/regenerating liver in mice. In this study, we studied the role of miR-27b in ASCs and their regenerative potential after partial liver resection in rats. ASCs transfected with control siRNA or miR-27b were intravenously injected into autologous rats undergoing 70% partial hepatectomy (PH). Our data showed that the regenerative capacities of ASCs with overexpressed miR-27b were significantly higher compared with control ASCs. However, the enhanced regeneration, hepatic differentiation, and suppressed liver inflammation, as well as fibrotic activity, were significantly reverted by ZnPP coadministration (heme oxygenase-1 [HO-1] inhibitor) indicating an important role of HO-1 in the regenerating and cytoprotective activities of miR-27b–transfected ASCs. We demonstrated that administration of autologous ASCs overexpressed with miR-27b enhances rapid and early liver regeneration and, importantly, preserves function after PH. The ASCs with miR-27b overexpression might offer a viable therapeutic option to facilitate rapid recovery after liver resection. |
| Databáze: | OpenAIRE |
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