Detection of charged proteins by means of impedance measurements
| Autor: | Jan Feijen, J.P. Rispens, J. Kruise, J.R. Haak, David N. Reinhoudt, F.J.B. Kremer, Piet Bergveld, D.A.J. Starmans |
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| Přispěvatelé: | Faculty of Science and Technology, Biomaterials Science and Technology |
| Jazyk: | angličtina |
| Rok vydání: | 1992 |
| Předmět: |
Chemistry
musculoskeletal neural and ocular physiology Metals and Alloys Analytical chemistry Electrolyte Condensed Matter Physics Surfaces Coatings and Films Electronic Optical and Magnetic Materials Ion chemistry.chemical_compound Membrane Electrical resistance and conductance Fixed charge Materials Chemistry Electrical and Electronic Engineering ISFET Lysozyme Instrumentation Electrical impedance |
| Zdroj: | Sensors and actuators. B: Chemical, 6(1-3), 101-105. Elsevier |
| ISSN: | 0925-4005 |
| Popis: | The electrical resistance of a membrane containing charged proteins is a function of both concentration and mobility of the electrolyte ions present in the membrane, and thus it depends on the Donnan effect. We were able to determine the resistance of such a membrane deposited on an ISFET, by means of a simple a.c. impedance measurement. As an example a membrane containing cross-linked lysozyme was used. We found the membrane resistance to be inversely proportional to the fixed charge density, in good agreement with the theoretical description based on the Donnan theory. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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