Characterization of liver cytokeratin as a major target antigen of anti-SLA antibodies
| Autor: | A. Kyriatsoulis, Michael Manns, AW Lohse, G. Gerken, B. Wächter, K H Meyer zum Büschenfelde |
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| Rok vydání: | 1990 |
| Předmět: |
Anti-nuclear antibody
Immunoblotting Fluorescent Antibody Technique Enzyme-Linked Immunosorbent Assay Immunofluorescence Chromatography Affinity Cytokeratin Antigen Affinity chromatography medicine Humans Antigens Autoantibodies Hepatology medicine.diagnostic_test biology fungi S100 Proteins Antibodies Monoclonal Virology Primary and secondary antibodies Immunohistochemistry Liver Monoclonal biology.protein Keratins Electrophoresis Polyacrylamide Gel Antibody |
| Zdroj: | Journal of hepatology. 11(2) |
| ISSN: | 0168-8278 |
| Popis: | Anti-SLA antibodies characterize a newly defined subgroup of patients with autoimmune chronic active hepatitis. The aim of the present study was the immunochemical characterization of the target antigen(s) of anti-SLA antibodies. Anti-SLA-positive sera were found to contain high titres of anti-cytokeratin antibodies. In immunoblotting analyses with 100 000 × g supernatants of human liver homogenates (S-100) these sera recognized various proteins with a molecular mass of 40–60 kDa. These proteins were also recognized by monoclonal anti-cytokeratin antibodies. Two-dimensional co-electrophoresis and immunoblotting analysis of S-100 and liver cytokeratins showed that anti-SLA antibodies were primarily directed against cytokeratins, particularly against liver cytokeratin types 8 and 18. This was supported by affinity chromatography, immunofluorescence and absorption methods using anti-SLA sera. |
| Databáze: | OpenAIRE |
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