Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia
| Autor: | Rhesi Kristiana, Christel Marty, Diah Ayuningrum, Muhammad Hanafi, I. Wayan Mudianta, Agus Sabdono, Ocky Karna Radjasa, Gilles Bedoux, Gerard Pals, Agus Trianto, Laure Taupin, Meezan Ardhanu Asagabaldan, Nathalie Bourgougnon |
|---|---|
| Přispěvatelé: | Human genetics |
| Rok vydání: | 2020 |
| Předmět: |
medicine.drug_class
Phyllidia varicosa Antibiotics lcsh:Medicine Virgibacillus salarius Pseudoalteromonas rubra medicine.disease_cause Microbiology 01 natural sciences General Biochemistry Genetics and Molecular Biology Anti-MRSA activity 03 medical and health sciences medicine Cytotoxicity 030304 developmental biology 0303 health sciences Nudibranch Aquatic and Marine Chemistry Strain (chemistry) biology 010405 organic chemistry Chemistry General Neuroscience lcsh:R Antibiotic Biodiversity General Medicine biology.organism_classification HSV-1 0104 chemical sciences 3. Good health Infectious Diseases Staphylococcus aureus Vero cell General Agricultural and Biological Sciences Bacteria Biotechnology |
| Zdroj: | PeerJ, Vol 8, p e8093 (2020) Kristiana, R, Bedoux, G, Pals, G, Wayan Mudianta, I, Taupin, L, Marty, C, Asagabaldan, M A, Ayuningrum, D, Trianto, A, Bourgougnon, N, Radjasa, O K, Sabdono, A & Hanafi, M 2020, ' Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia ', PeerJ, vol. 2020, no. 1, e8093 . https://doi.org/10.7717/peerj.8093 PeerJ, 2020(1):e8093. PeerJ Inc. PeerJ |
| ISSN: | 2167-8359 |
| DOI: | 10.7717/peerj.8093 |
| Popis: | The aims of this work are to isolate bacterial symbionts from nudibranchs and subsequently to determine anti-Methicillin resistant Staphylococcus aureus (MRSA), cytotoxicity and anti-Herpes simplex virus type 1 (HSV-1) activities of bio compounds. A total of 15 species of nudibranchs were collected from Karimunjawa and five species from Bali, respectively. A total of 245 bacteria isolates were obtained. The anti-MRSA activity screening activity indicated two active bacteria. Ethyl acetate extracts from supernatants, indicating extracelullar compounds, showed an inhibition zone against MRSA at concentrations of 500–1,000 µg/ml. DNA sequence analysis showed that the strain KJB-07 from Phyllidia coelestis was closely related to Pseudoalteromonas rubra, whereas the strain NP31-01 isolated from Phyllidia varicosa was closely related to Virgibacillus salarius. The extract of Pseudoalteromonas rubra was cytotoxic to Vero cells at a concentration of 75 µg/ml. The extract of V. salarius presented no cytotoxicity at concentrations of 5–1,000 µg/ml. No anti HSV-1 was observed for both isolated bacteria. This is the first study describing research on anti-MRSA, cytotoxicity and anti HSV-1 activity of bacterial symbionts from the viscera of nudibranch. Compounds produced by Pseudoalteromonas rubra and V. salarius, had potential anti-MRSA activity. However, only extracts from Pseudoalteromonas rubra showed cytotoxic effects on Vero cells. Three compounds were identified by LC/MS after purification from culture supernatant. |
| Databáze: | OpenAIRE |
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