International Lp(a) standardization
| Autor: | O. Henderson, M. Rosseneu, C. Labeur |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Analysis of Variance
Chromatography Standardization Chemistry Data Collection International Cooperation Organic Chemistry International survey Cell Biology Reference Standards Biochemistry Ria radioimmunoassay Primary standard ELISA - Enzyme-linked immunosorbent assay Statistics Humans Laboratories Molecular Biology Reference standards Blood Chemical Analysis Lipoprotein(a) |
| Zdroj: | Chemistry and Physics of Lipids. :265-270 |
| ISSN: | 0009-3084 |
| DOI: | 10.1016/0009-3084(94)90146-5 |
| Popis: | Two international surveys for Lp(a) measurements were organized from 1989 to 1991. The results of the first survey led to the conclusion that the lack of a common primary standard was the main cause of the large inter-laboratory variation observed. No major effects of techniques or antisera were observed. The same findings were confirmed during the second survey, which was extended to include more samples and a larger number of participants. During the second survey, no consistent effect due to freezing or lyophilization could be demonstrated, although there was a trend towards lower Lp(a) values in lyophilized samples. The inter- and intra-laboratory coefficients of variation did not vary significantly for the different Lp(a) phenotypes, and variability was comparable for lyophilized, liquid and frozen materials. Large intra-assay coefficients of variation were observed during both surveys. Results obtained in different laboratories using the same commercial reagents and standards also showed a large variation. These initial results demonstrate that the lack of a primary standard and poor assay precision are the main factors responsible for the high inter-laboratory variation observed during these surveys. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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