Spectroscopic photoacoustic imaging of cervical tissue composition in excised human samples

Autor: Roberto Romero, Sonia S. Hassan, Edgar Hernandez-Andrade, Yan Yan, Aneesha Varrey, Richard Hsu, Ali Alhousseini, Mohammad Mehrmohammadi, Alpana Garg, Maryam Basij
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Photoacoustic Spectroscopy
medicine.medical_treatment
Maternal Health
H&E stain
Cervix Uteri
Biochemistry
Cervix
Diagnostic Radiology
chemistry.chemical_compound
Spectrum Analysis Techniques
Pregnancy
Ultrasound Imaging
Medicine and Health Sciences
Medicine
reproductive and urinary physiology
Multidisciplinary
Radiology and Imaging
Ultrasound
Obstetrics and Gynecology
medicine.anatomical_structure
Optical Equipment
Gestation
Engineering and Technology
Female
Collagen
Anatomy
Genital Anatomy
Research Article
Adult
Diagnostic Imaging
Histology
Imaging Techniques
Science
Equipment
Research and Analysis Methods
Photoacoustic Techniques
Diagnostic Medicine
Humans
Sirius Red
Hysterectomy
business.industry
Lasers
Reproductive System
Biology and Life Sciences
Proteins
medicine.disease
Cross-Sectional Studies
chemistry
Women's Health
business
Nuclear medicine
Collagens
Zdroj: PLoS ONE
PLoS ONE, Vol 16, Iss 3, p e0247385 (2021)
ISSN: 1932-6203
Popis: ObjectiveCervical remodeling is an important component in determining the pathway of parturition; therefore, assessing changes in cervical tissue composition may provide information about the cervix’s status beyond the measurement of cervical length. Photoacoustic imaging is a non-invasive ultrasound-based technology that captures acoustic signals emitted by tissue components in response to laser pulses. This optical information allows for the determination of the collagen-to-water ratio (CWR). The purpose of this study was to compare the CWR evaluated by using spectroscopic photoacoustic (sPA) imaging in cervical samples obtained from pregnant and non-pregnant women.MethodsThis cross-sectional study comprised cervical biopsies obtained at the time of hysterectomy (n = 8) and at the scheduled cesarean delivery in pregnant women at term who were not in labor (n = 8). The cervical CWR was analyzed using a fiber-optic light-delivery system integrated to an ultrasound probe. The photoacoustic signals were acquired within the range of wavelengths that cover the peak absorption of collagen and water. Differences in the CWR between cervical samples from pregnant and non-pregnant women were analyzed. Hematoxylin and eosin and Sirius Red stains were used to compare the collagen content of cervical samples in these two groups.ResultsEight cervix samples were obtained after hysterectomy, four from women ≤41 years of age and four from women ≥43 years of age; all cervical samples (n = 8) from pregnant women were obtained after 37 weeks of gestation at the time of cesarean section. The average CWR in cervical tissue samples from pregnant women was 18.7% (SD 7.5%), while in samples from non-pregnant women, it was 55.0% (SD 20.3%). There was a significantly higher CWR in the non-pregnant group compared to the pregnant group with a p-value ConclusionThe proposed bimodal ultrasound and sPA imaging system can provide information on the biochemical composition of cervical tissue in pregnant and non-pregnant women. Photoacoustic imaging showed a higher collagen content in cervical samples from non-pregnant women as compared to those from pregnant women, which matched with the histological analysis. This novel imaging method envisions a new potential for a sensitive diagnostic tool in the evaluation of cervical tissue composition.
Databáze: OpenAIRE
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