Efficacy of A/H1N1/2009 split inactivated influenza A vaccine (GC1115) in mice and ferrets
| Autor: | Su-Jin Park, Young Ki Choi, Suk-Hoon Ha, Norbert John Robles, Min-Suk Song, Hae Jung Han, Han Yeul Byun |
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| Rok vydání: | 2018 |
| Předmět: |
Respiratory System
Dose-Response Relationship Immunologic Antibodies Viral Applied Microbiology and Biotechnology Microbiology Injections Intramuscular Virus Body Temperature 03 medical and health sciences Mice Immunogenicity Vaccine Influenza A Virus H1N1 Subtype Antigen Adjuvants Immunologic Orthomyxoviridae Infections Medicine Animals Seroconversion Lung 030304 developmental biology 0303 health sciences Mice Inbred BALB C biology 030306 microbiology business.industry Immunogenicity Body Weight Vaccination Ferrets General Medicine Hemagglutination Inhibition Tests Viral Load Virology Survival Rate Titer Disease Models Animal Immunization Cough Vaccines Inactivated Influenza Vaccines biology.protein Antibody business |
| Zdroj: | Journal of microbiology (Seoul, Korea). 57(2) |
| ISSN: | 1976-3794 |
| Popis: | To evaluate the efficacy of a non-adjuvant A/H1N1/2009 influenza A vaccine (GC1115), we demonstrated the immunogenicity and protective efficacy of GC1115 in mouse and ferret models. The immunogenicity of GC1115 was confirmed after intramuscular administration of 1.875, 3.75, 7.5, and 15 μg hemagglutinin antigen (HA) in mice and 7.5, 15, and 30 μg HA in ferrets at 3-week intervals. A single immunization with GC1115 at HA doses > 7.5 μg induced detectable seroconversion in most mice, and all mice given a second dose exhibited high antibody responses in a dose-dependent manner. The mice in the mock (PBS) and 1.875 μg HA immunized groups succumbed by 13 days following A/California/ 04/09 infection, while all mice in groups given more than 3.75 μg HA were protected from lethal challenge with the A/California/04/09 virus. In ferrets, although immunization with even a single dose of 15 or 30 μg of HA induced detectable HI antibodies, all ferrets given two doses of vaccine seroconverted and exhibited HI titers greater than 80 units. Following challenge with A/California/04/09, the mock (PBS) immunized ferrets showed influenza-like clinical symptoms, such as increased numbers of coughs, elevated body temperature, and body weight loss, for 7 days, while GC1115- immunized ferrets showed attenuated clinical symptoms only for short time period (3–4 days). Further, GC1115-immunized ferrets displayed significantly lower viral titers in the upper respiratory tract (nasal cavity) than the mock vaccinated group in a dose-dependent manner. Taken together, this study demonstrates the immunogenicity and protective efficacy of GC1115 as a non-adjuvanted vaccine. |
| Databáze: | OpenAIRE |
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