Deleted in malignant brain tumor 1 is secreted in the oviduct and involved in the mechanism of fertilization in equine and porcine species
| Autor: | Ghylène Goudet, Sylvie Canepa, B. Ambruosi, Salvatore Desantis, Catherine Robbe-Masselot, Carla Moros Nicolás, Géraldine Pascal, Uffe Holmskov, Gianluca Accogli, Jan Mollenhauer, Olivier Vidal, Philippe Monget, Cécile Douet |
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| Přispěvatelé: | Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Dipartimento dell'Emergenze e Trapianti di Organo, Università degli studi di Bari Aldo Moro (UNIBA), Facultad de Medicina, Universidad de Murcia, Department of Cardiovascular and Renal Research, University of Southern Denmark (SDU), Department of Cardiovascular and Renal Research, Lundbeckfonden Center of Excellence NanoCAN, Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut Français du Cheval et de l'Equitation (IFCE), Université François Rabelais de Tours, Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Università degli studi di Bari, Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Università degli studi di Bari Aldo Moro = University of Bari Aldo Moro (UNIBA), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
Embryology Bodily Secretions Cytoplasm Sus scrofa Oviducts Zona Pellucida/metabolism 0302 clinical medicine Endocrinology Human fertilization Cumulus Cells/physiology Zona pellucida Bodily Secretions/metabolism Recombinant Proteins/metabolism 0303 health sciences 030219 obstetrics & reproductive medicine Cumulus Cells Membrane Glycoproteins medicine.diagnostic_test Obstetrics and Gynecology Oocytes/cytology Spermatozoa Recombinant Proteins Cytoplasm/metabolism Protein Transport medicine.anatomical_structure Immunohistochemistry Oviduct Oviducts/cytology Female Antibodies/metabolism endocrine system [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] animal structures Membrane Glycoproteins/antagonists & inhibitors Estrous Cycle Fertilization in Vitro Biology Estrous Cycle/metabolism Immunofluorescence Antibodies Andrology 03 medical and health sciences Western blot medicine Animals Horses Zona Pellucida 030304 developmental biology Sperm-Ovum Interactions urogenital system Embryogenesis Mucins Cell Biology Molecular biology Reproductive Medicine Mucins/antagonists & inhibitors Spermatozoa/cytology Oocytes Fertilization in Vitro/veterinary |
| Zdroj: | Reproduction Reproduction, BioScientifica, 2013, 146 (2), pp.119-133. ⟨10.1530/REP-13-0007⟩ Ambruosi, B, Accogli, G, Douet, C, Canepa, S, Pascal, G, Monget, P, Moros Nicolas, C, Holmskov, U, Mollenhauer, J, Robbe-Masselot, C, Vidal, O, Desantis, S & Goudet, G 2013, ' Deleted in malignant brain tumor 1 is secreted in the oviduct and involved in the mechanism of fertilization in equine and porcine species ', Reproduction, vol. 146, no. 2, pp. 119-133 . https://doi.org/10.1530/REP-13-0007 Reproduction, 2013, 146 (2), pp.119-133. ⟨10.1530/REP-13-0007⟩ |
| ISSN: | 1470-1626 1741-7899 |
| DOI: | 10.1530/REP-13-0007⟩ |
| Popis: | Remerciements : Plate-formes CIRE et PIC, INRA, UMR PRC Physiologie de la Reproduction et des Comportements, 37380 Nouzilly; Oviductal environment affects preparation of gametes for fertilization, fertilization itself, and subsequent embryonic development. The aim of this study was to evaluate the effect of oviductal fluid and the possible involvement of deleted in malignant brain tumor 1 (DMBT1) on IVF in porcine and equine species that represent divergent IVF models. We first performed IVF after pre-incubation of oocytes with or without oviductal fluid supplemented or not with antibodies directed against DMBT1. We showed that oviductal fluid induces an increase in the monospermic fertilization rate and that this effect is canceled by the addition of antibodies, in both porcine and equine species. Moreover, pre-incubation of oocytes with recombinant DMBT1 induces an increase in the monospermic fertilization rate in the pig, confirming an involvement of DMBT1 in the fertilization process. The presence of DMBT1 in the oviduct at different stages of the estrus cycle was shown by western blot and confirmed by immunohistochemical analysis of ampulla and isthmus regions. The presence of DMBT1 in cumulus-oocyte complexes was shown by western blot analysis, and the localization of DMBT1 in the zona pellucida and cytoplasm of equine and porcine oocytes was observed using immunofluorescence analysis and confocal microscopy. Moreover, we showed an interaction between DMBT1 and porcine spermatozoa using surface plasmon resonance studies. Finally, a bioinformatic and phylogenetic analysis allowed us to identify the DMBT1 protein as well as a DMBT1-like protein in several mammals. Our results strongly suggest an important role of DMBT1 in the process of fertilization. |
| Databáze: | OpenAIRE |
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