A new unique form of microRNA from human heart, microRNA-499c, promotes myofibril formation and rescues cardiac development in mutant axolotl embryos
| Autor: | Jessica Meyer, Ashley Moses, Larry F. Lemanski, Michael C Hanna, Andrei Kochegarov, William Lian |
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| Jazyk: | angličtina |
| Předmět: |
Myofibrillogenesis
Endocrinology Diabetes and Metabolism Mutant Clinical Biochemistry Sequence alignment Tropomyosin 030204 cardiovascular system & hematology Cardiac mutant axolotl 03 medical and health sciences 0302 clinical medicine Organ Culture Techniques Myofibrils Axolotl microRNA Animals Humans Pharmacology (medical) Molecular Biology 030304 developmental biology Biochemistry medical 0303 health sciences biology Heart development Research Myocardium Biochemistry (medical) RNA Human embryonic heart RNA Gene Expression Regulation Developmental Heart General Medicine Cell Biology biology.organism_classification musculoskeletal system Molecular biology Myocardial Contraction Ambystoma mexicanum MicroRNAs Mutation embryonic structures Myofibril |
| Zdroj: | Journal of Biomedical Science |
| ISSN: | 1423-0127 |
| DOI: | 10.1186/1423-0127-20-20 |
| Popis: | Background A recessive mutation “c” in the Mexican axolotl, Ambystoma mexicanum, results in the failure of normal heart development. In homozygous recessive embryos, the hearts do not have organized myofibrils and fail to beat. In our previous studies, we identified a noncoding Myofibril-Inducing RNA (MIR) from axolotls which promotes myofibril formation and rescues heart development. Results We randomly cloned RNAs from fetal human heart. RNA from clone #291 promoted myofibril formation and induced heart development of mutant axolotls in organ culture. This RNA induced expression of cardiac markers in mutant hearts: tropomyosin, troponin and α-syntrophin. This cloned RNA matches in partial sequence alignment to human microRNA-499a and b, although it differs in length. We have concluded that this cloned RNA is unique in its length, but is still related to the microRNA-499 family. We have named this unique RNA, microRNA-499c. Thus, we will refer to this RNA derived from clone #291 as microRNA-499c throughout the rest of the paper. Conclusions This new form, microRNA-499c, plays an important role in cardiac development. |
| Databáze: | OpenAIRE |
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