Prevalence of Chlamydia trachomatis Genotypes in Men Who Have Sex with Men and Men Who Have Sex with Women Using Multilocus VNTR Analysis-ompA Typing in Guangzhou, China

Autor: Heping Zheng, Xuqi Ren, Jiangli Ou, Yaohua Xue, Xingzhong Wu, Bin Yang, Yinyuan Lan, Jinmei Huang, Sanmei Tang, Shujie Huang, Xiaolin Qin, Weiying Zeng
Rok vydání: 2015
Předmět:
0301 basic medicine
Male
lcsh:Medicine
Gene Expression
Gene Sequencing
Chlamydia trachomatis
Artificial Gene Amplification and Extension
medicine.disease_cause
Pathology and Laboratory Medicine
Polymerase Chain Reaction
Men who have sex with men
Geographical Locations
0302 clinical medicine
Sequencing techniques
Epidemiology
Genotype
Medicine and Health Sciences
030212 general & internal medicine
DNA sequencing
Chlamydia
lcsh:Science
Phylogeny
Multidisciplinary
Geography
respiratory system
Middle Aged
Bacterial Pathogens
Phylogeography
Sexual Partners
Infectious Diseases
Biogeography
Tandem Repeat Sequences
Medical Microbiology
Female
Pathogens
Bacterial Outer Membrane Proteins
Research Article
Adult
medicine.medical_specialty
China
Genotyping
Asia
Adolescent
030106 microbiology
Sexually Transmitted Diseases
Men WHO Have Sex with Men
Biology
Multiple Loci VNTR Analysis
Research and Analysis Methods
Microbiology
03 medical and health sciences
Urethra
medicine
Genetics
Humans
Typing
Homosexuality
Male
Molecular Biology Techniques
Microbial Pathogens
Molecular Biology
Evolutionary Biology
Bacteria
Population Biology
lcsh:R
Ecology and Environmental Sciences
Rectum
Organisms
Sexual Preferences
Biology and Life Sciences
Chlamydia Infections
bacterial infections and mycoses
Virology
People and Places
Earth Sciences
Multilocus sequence typing
bacteria
lcsh:Q
Population Groupings
Population Genetics
Multilocus Sequence Typing
Zdroj: PLoS ONE
PLoS ONE, Vol 11, Iss 7, p e0159658 (2016)
ISSN: 1932-6203
Popis: Background Chlamydia trachomatis is one of the most prevalent bacterial sexually transmitted infection in China. Although C. trachomatis genotypes can be discriminated by outer membrane protein gene (ompA) sequencing, currently available methods have limited resolutions. This study used a high-resolution genotyping method, namely, multilocus variable number tandem-repeat analysis with ompA sequencing (MLVA)-ompA, to investigate the local epidemiology of C. trachomatis infections among men who have sex with men (MSM) and men who have sex with women (MSW) attending a sexually transmitted diseases (STD) clinic in Guangzhou, China. Methods Rectal specimens from MSM and urethral specimens from MSW were collected between January 2013 and July 2014 at the Guangdong Provincial Center STD clinic. The specimens were sent to the laboratory for analyses. All specimens that were tested positive for C. trachomatis by the commercial nucleic acid amplification tests were genotyped by MLVA-ompA. Results Fifty-one rectal specimens from MSM and 96 urethral specimens from MSW were identified with C. trachomatis. One hundred and forty-four of the 147 specimens were fully genotyped by MLVA-ompA. Rectal specimens from MSM were divided into four ompA genotypes and urethral specimens from MSW into nine genotypes. No mixed infections were found among all specimens. The most frequent genotypes were D, G, J, E and F. All specimens were further divided into 46 types after ompA genotyping was combined with MLVA. Genotypes D-8.7.1 and G-3.4a.3 were the most frequent among MSM, whereas genotypes D-3.4a.4, E-8.5.1, F-8.5.1, and J-3.4a.2 were the most frequent subtypes among MSW. The discriminatory index D was 0.90 for MLVA, 0.85 for ompA, and 0.95 for MLVA-ompA. Conclusions The most prevalent MLVA-ompA genotypes were significantly different between MSM and MSW from Guangzhou, China. Moreover, MLVA-ompA represented a more favorable degree of discrimination than ompA and could be a reliable complement for ompA for the routine subtypes of C. trachomatis.
Databáze: OpenAIRE
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