Prevalence of Chlamydia trachomatis Genotypes in Men Who Have Sex with Men and Men Who Have Sex with Women Using Multilocus VNTR Analysis-ompA Typing in Guangzhou, China
Autor: | Heping Zheng, Xuqi Ren, Jiangli Ou, Yaohua Xue, Xingzhong Wu, Bin Yang, Yinyuan Lan, Jinmei Huang, Sanmei Tang, Shujie Huang, Xiaolin Qin, Weiying Zeng |
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Rok vydání: | 2015 |
Předmět: |
0301 basic medicine
Male lcsh:Medicine Gene Expression Gene Sequencing Chlamydia trachomatis Artificial Gene Amplification and Extension medicine.disease_cause Pathology and Laboratory Medicine Polymerase Chain Reaction Men who have sex with men Geographical Locations 0302 clinical medicine Sequencing techniques Epidemiology Genotype Medicine and Health Sciences 030212 general & internal medicine DNA sequencing Chlamydia lcsh:Science Phylogeny Multidisciplinary Geography respiratory system Middle Aged Bacterial Pathogens Phylogeography Sexual Partners Infectious Diseases Biogeography Tandem Repeat Sequences Medical Microbiology Female Pathogens Bacterial Outer Membrane Proteins Research Article Adult medicine.medical_specialty China Genotyping Asia Adolescent 030106 microbiology Sexually Transmitted Diseases Men WHO Have Sex with Men Biology Multiple Loci VNTR Analysis Research and Analysis Methods Microbiology 03 medical and health sciences Urethra medicine Genetics Humans Typing Homosexuality Male Molecular Biology Techniques Microbial Pathogens Molecular Biology Evolutionary Biology Bacteria Population Biology lcsh:R Ecology and Environmental Sciences Rectum Organisms Sexual Preferences Biology and Life Sciences Chlamydia Infections bacterial infections and mycoses Virology People and Places Earth Sciences Multilocus sequence typing bacteria lcsh:Q Population Groupings Population Genetics Multilocus Sequence Typing |
Zdroj: | PLoS ONE PLoS ONE, Vol 11, Iss 7, p e0159658 (2016) |
ISSN: | 1932-6203 |
Popis: | Background Chlamydia trachomatis is one of the most prevalent bacterial sexually transmitted infection in China. Although C. trachomatis genotypes can be discriminated by outer membrane protein gene (ompA) sequencing, currently available methods have limited resolutions. This study used a high-resolution genotyping method, namely, multilocus variable number tandem-repeat analysis with ompA sequencing (MLVA)-ompA, to investigate the local epidemiology of C. trachomatis infections among men who have sex with men (MSM) and men who have sex with women (MSW) attending a sexually transmitted diseases (STD) clinic in Guangzhou, China. Methods Rectal specimens from MSM and urethral specimens from MSW were collected between January 2013 and July 2014 at the Guangdong Provincial Center STD clinic. The specimens were sent to the laboratory for analyses. All specimens that were tested positive for C. trachomatis by the commercial nucleic acid amplification tests were genotyped by MLVA-ompA. Results Fifty-one rectal specimens from MSM and 96 urethral specimens from MSW were identified with C. trachomatis. One hundred and forty-four of the 147 specimens were fully genotyped by MLVA-ompA. Rectal specimens from MSM were divided into four ompA genotypes and urethral specimens from MSW into nine genotypes. No mixed infections were found among all specimens. The most frequent genotypes were D, G, J, E and F. All specimens were further divided into 46 types after ompA genotyping was combined with MLVA. Genotypes D-8.7.1 and G-3.4a.3 were the most frequent among MSM, whereas genotypes D-3.4a.4, E-8.5.1, F-8.5.1, and J-3.4a.2 were the most frequent subtypes among MSW. The discriminatory index D was 0.90 for MLVA, 0.85 for ompA, and 0.95 for MLVA-ompA. Conclusions The most prevalent MLVA-ompA genotypes were significantly different between MSM and MSW from Guangzhou, China. Moreover, MLVA-ompA represented a more favorable degree of discrimination than ompA and could be a reliable complement for ompA for the routine subtypes of C. trachomatis. |
Databáze: | OpenAIRE |
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