Efficacy of Antiretroviral Agents against Murine Replication-Competent Retrovirus Infection in Human Cells
| Autor: | Sharon K. Powell, Edward Otto, Michele Kaloss, Scott Brazinski, Moria Artlip, Gerard J. McGarrity, Russette M. Lyons |
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| Rok vydání: | 1999 |
| Předmět: |
viruses
Genetic Vectors Immunology Indinavir Virus Replication Microbiology Cell Line Zidovudine Retrovirus Virology medicine Humans Protease inhibitor (pharmacology) biology Nucleoside analogue Stavudine Gene Transfer Techniques Gene Therapy Genetic Therapy biology.organism_classification Leukemia Virus Murine Didanosine Viral replication Insect Science Reverse Transcriptase Inhibitors Replication Competent Retrovirus medicine.drug |
| Zdroj: | Journal of Virology. 73:8813-8816 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.73.10.8813-8816.1999 |
| Popis: | Retroviral vectors for gene therapy are designed to minimize the occurrence of replication-competent retrovirus (RCR); nonetheless, it is possible that a vector-derived RCR could establish an infection in a patient. Since the efficacy of antiretroviral agents can be impacted by interactions between virus, host cell, and drug, five commonly used antiretroviral drugs were evaluated for their abilities to inhibit the replication of a murine leukemia virus (MLV)-derived RCR in human cells. The results obtained indicate that the combination of nucleoside analogs zidovudine and dideoxyinosine with the protease inhibitor indinavir effectively inhibits MLV-derived RCR replication in three human cell lines. In addition, MLV-derived RCR was found to be inherently resistant to the nucleoside analogs lamivudine and stavudine, suggesting that mutations conferring resistance to nucleoside analogs in human immunodeficiency virus type 1 have the same effect even in an alternative viral backbone. |
| Databáze: | OpenAIRE |
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