Mesenchymal stem cell transplantation into the spinal cord of healthy adult horses undergoing cervical ventral interbody fusion
| Autor: | Inga-Catalina Cruz Benedetti, Nathalie Saulnier, Stéphane Maddens, Olivier M. Lepage, Cécile De Guio, Isabelle Desjardins, Elaine M. Carpenter, Barrie D. Grant, Isé François |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
medicine.medical_specialty Ataxia 040301 veterinary sciences Mesenchymal Stem Cell Transplantation law.invention 0403 veterinary science Intramedullary rod 03 medical and health sciences Postoperative Complications 0302 clinical medicine law medicine Animals Horses Cervical canal General Veterinary medicine.diagnostic_test business.industry Mesenchymal stem cell Endoscopy 04 agricultural and veterinary sciences Spinal cord Surgery Transplantation Dissection Spinal Fusion medicine.anatomical_structure Fluoroscopy 030220 oncology & carcinogenesis Cervical Vertebrae Feasibility Studies Female Horse Diseases medicine.symptom business Spinal Cord Compression |
| Zdroj: | Veterinary Surgery. 50:1107-1116 |
| ISSN: | 1532-950X 0161-3499 |
| Popis: | Objective To determine the feasibility of umbilical cord-derived mesenchymal stem cell (UC-MSC) transplantation into the cervical spinal cord of horses by using fluoroscopy with or without endoscopic guidance and to evaluate the neurological signs and tissue reaction after injection. Study design Experimental study. Animals Eight healthy adult horses with no clinical signs of neurological disease. Methods After cervical ventral interbody fusion (CVIF), ten million fluorescently labeled allogeneic UC-MSC were injected into the spinal cord under endoscopic and fluoroscopic guidance (n = 5) or fluoroscopic guidance only (n = 3). Postoperative neurological examinations were performed, and horses were humanely killed 48 hours (n = 4) or 14 days (n = 4) postoperatively. Spinal tissues were examined after gross dissection and with bright field and fluorescent microscopy. Results Needle endoscopy of the cervical canal by ventral approach was associated with intraoperative spinal cord puncture (2/5) and postoperative ataxia (3/5). No intraoperative complications occurred, and one (1/3) horse developed ataxia with cell transplantation under fluoroscopy alone. Umbilical cord-derived MSC were associated with small vessels and detected up to 14 days in the spinal cord. Demyelination was observed in six of eight cases. Conclusion Fluoroscopically guided intramedullary UC-MSC transplantation during CVIF avoids spinal cord trauma and decreases risk of ataxia from endoscopy. Umbilical cord-derived MSC persist in the spinal cord for up to 14 days. Cell injection promotes angiogenesis and induces demyelination of the spinal tissue. Clinical significance Umbilical cord-derived MSC transplantation into the spinal cord during CVIF without endoscopy is recommended for future evaluation of cell therapy in horses affected by cervical vertebral compressive myelopathy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text