A MICROMETHOD FOR THE DETERMINATION OF SOLASODINE INSOLANUMSPECIES
| Autor: | S. W. Amin, Saad M. Khafagy, R. Hassanin |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Pharmacology
Plants Medicinal Chromatography biology Plant Extracts Elution Alkaloid Organic Chemistry Pharmaceutical Science biology.organism_classification Solasodine Analytical Chemistry chemistry.chemical_compound Hydrolysis Alkaloids Aglycone Species Specificity Complementary and alternative medicine chemistry Drug Discovery Molecular Medicine Glycosides Absorption (chemistry) Solanum Benzene |
| Zdroj: | Planta Medica. 21:139-141 |
| ISSN: | 1439-0221 0032-0943 |
| DOI: | 10.1055/s-0028-1099533 |
| Popis: | A micromethod is described for the determination of solasodine alkaloid in small samples of leaves and berries of Solanum species grown in Egypt. The glyco–alkaloid is extracted and hydrolyzed by the usual methods. The aglycone is purified and separated by T.L.C. using chloroform–ethanol on silicagel G plates. The purified eluted aglycone is complexed with bromothymol–blue and the colour complex is extracted into benzene and determined colorimetrically using solasodine as a standard. The colour complex is stable for 45 minutes and obeys Beer's low in concentrations from 10 to 200 μg and has its maximum absorption at 400 mμ. |
| Databáze: | OpenAIRE |
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