Autonomous contractile activity in the isolated rat bladder is modulated by a TRPV1 dependent mechanism
| Autor: | Dieter Ost, Dirk De Ridder, Thomas Gevaert, Joachim Vandepitte, Bernd Nilius |
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| Rok vydání: | 2006 |
| Předmět: |
Agonist
medicine.medical_specialty Carbachol medicine.drug_class Urology Neurotoxins Urinary Bladder Resiniferatoxin TRPV1 TRPV Cation Channels Stimulation Cholinergic Agonists Contractility chemistry.chemical_compound Internal medicine Muscarinic acetylcholine receptor medicine Animals Rats Wistar Urinary Bladder Neurogenic Urinary bladder business.industry Electric Stimulation Rats Urodynamics Endocrinology medicine.anatomical_structure chemistry Anesthesia Female Neurology (clinical) Diterpenes business medicine.drug Muscle Contraction |
| Zdroj: | Neurourology and urodynamics. 26(3) |
| ISSN: | 0733-2467 |
| Popis: | Aims Resiniferatoxin (RTX), a vanilloid compound and agonist of the transient receptor potential channel 1 (TRPV1), is known for its beneficial effects on neurogenic detrusor overactivity. The mainstream rationale for its use is the desensitization of TRPV1 on sensory bladder afferents. However, recent findings showed that TRPV1 is present in other cell types in the bladder. To eliminate the effects of RTX on spinal and central neural circuits, we investigated autonomous contractility in normal and neurogenic rat bladders after treatment with RTX. Methods Female Wistar rats were made paraplegic at vertebral level T8–T9. Animals were intravesically pre-treated with vehicle (ethanol 5%) or RTX (100 nM) and sacrificed after 72 hr. Each bladder was excised and placed in a heated organ bath, where intravesical pressures were measured. Effects on contractile parameters of intravesical volume load, the non-selective muscarinic receptor agonist carbachol (CA) and electrical stimulation (ES) of nerves were studied in both groups. Results In RTX-treated normal bladders we found shorter contractions with higher amplitude than in control bladders (P |
| Databáze: | OpenAIRE |
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