Long non-coding RNA SNHG8 enhances triple-negative breast cancer cell proliferation and migration by regulating the miR-335-5p/PYGO2 axis

Autor:	Lu Zheng, Jia Li, Xinhan Lei, Wanwan Li, Jianing Shi, Tong Tang, Shuai Zhang, Lei Zhang, Wenjun Jia, Jintao Qian, Yue Sun
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	QH301-705.5 Immunology PYGO2 Triple Negative Breast Neoplasms miR-335-5p Biology General Biochemistry Genetics and Molecular Biology SNHG8 Triple-negative breast cancer Cell Line Tumor Humans Gene silencing Small nucleolar RNA Biology (General) Ecology Evolution Behavior and Systematics Cell Proliferation Cell growth Research Applied Mathematics Intracellular Signaling Peptides and Proteins RNA biology.organism_classification Long non-coding RNA Up-Regulation Gene Expression Regulation Neoplastic MicroRNAs PHD finger Modeling and Simulation Cancer research RNA Long Noncoding General Agricultural and Biological Sciences Pygopus
Zdroj:	Biology Direct, Vol 16, Iss 1, Pp 1-10 (2021) Biology Direct
ISSN:	1745-6150
Popis:	Background Growing evidence has demonstrated that long non-coding RNAs (lncRNAs) can function as modulators in the development of triple-negative breast cancer (TNBC). However, the function of lncRNA small nucleolar RNA host gene 8 (SNHG8) in TNBC remains unclear. Therefore, our study aimed at investigating the role of SNHG8 in the proliferation and migration of TNBC cells. Methods SNHG8 expression was evaluated using RT-qPCR assay. Cell proliferation and migration were assessed by EdU, colony formation and Transwell assays. The levels of proteins related to EMT process were examined by western blot assay. The interaction among SNHG8, miR-335-5p and pygopus family PHD finger 2 (PYGO2) was detected by RIP assay, RNA pull down assay and luciferase reporter assay. Results SNHG8 expression was significantly up-regulated in TNBC cells. SNHG8 silencing obviously inhibited TNBC cell proliferation, migration and EMT process. Moreover, SNHG8 acted as a sponge to sequester miR-335-5p in TNBC cells. Besides, PYGO2 was proven as a target gene of miR-335-5p, and SNHG8 promoted TNBC cell proliferation, migration and EMT process through regulating miR-335-5p and PYGO2. Conclusions Totally, our study indicated that SNHG8 promoted TNBC cell proliferation and migration by regulating the miR-335-5p/PYGO2 axis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0547966df61a3b7a7ca0dd0ac0fc9325 https://doaj.org/article/4588354391c241fcbaaa2e4b1a981c82 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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