PEGylated lipid bilayer coated mesoporous silica nanoparticles co-delivery of paclitaxel and curcumin leads to increased tumor site drug accumulation and reduced tumor burden
Autor: | Huihao Xu, Jiahao Lin, Shuaiyu Wang, Zhongjie Liu, Yipeng Jin, Qian Wang, Kai Fan, Linna Zhao, Degui Lin, Yinian Tang, Jiafeng Gao |
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Rok vydání: | 2019 |
Předmět: |
Curcumin
Paclitaxel Cell Survival Surface Properties Drug Compounding Lipid Bilayers Pharmaceutical Science Mice Nude Antineoplastic Agents Breast Neoplasms 02 engineering and technology Pharmacology 030226 pharmacology & pharmacy Polyethylene Glycols Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Dogs Pharmacokinetics Nanocapsules In vivo Cell Line Tumor Distribution (pharmacology) Animals Humans Mice Inbred BALB C Chemistry Biological Transport Mesoporous silica 021001 nanoscience & nanotechnology Silicon Dioxide Controlled release Mitochondria Tumor Burden Drug Liberation Drug delivery Drug Therapy Combination Female 0210 nano-technology Lysosomes Porosity |
Zdroj: | European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences. 140 |
ISSN: | 1879-0720 |
Popis: | Homogeneous PEGylated lipid bilayer coated highly ordered MSNs (PLMSNs) which were systematically optimized and characterized to co-encapsulate paclitaxel (Tax) and curcumin (Cur) were verified to manifest prolonged and enhanced cytotoxic effect against canine breast cancer cells in our previous study. In this article, we took further study of the pharmacokinetic property, cellular uptake, subcellular localization, in vivo distribution and tumor accumulation ability, and treatment efficacy of the drug delivery system. The results revealed that the delivery system could significantly increase the AUC of two drugs, and the anti-tumor effect showed that both intravenous and intratumoral administration group better controlled the tumor weight than that of other groups (P .05), and the anti-tumor rates were 58.4% and 58.3% respectively. Cell uptake and localization study showed that PLMSNs could effectively carry drugs into cancer cells with sustained release characteristics. The subcellular localization of PLMSNs was mainly in lysosomes and mitochondria. In vivo fluorescence tracing results showed that PLMSNs could be effectively accumulated in the tumor site. The results revealed that the delivery system could effectively reduce the clinical dosage of drugs and reduce its toxic side effects, effectively carry drugs into cancer cells, and exhibit good targeting characteristics for breast cancer. |
Databáze: | OpenAIRE |
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