Comparative Chemical Profiling and Monacolins Quantification in Red Yeast Rice Dietary Supplements by 1H-NMR and UHPLC-DAD-MS
| Autor: | Stéphane Balayssac, Nathalie Martins-Froment, Myriam Malet-Martino, Véronique Gilard, Rabab Hachem, Robert Martino, Gaëtan Assemat |
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| Přispěvatelé: | Synthèse et Physico-Chimie de Molécules d'Intérêt Biologique (SPCMIB), Institut de Chimie de Toulouse (ICT-FR 2599), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Pharmaceutical Science
Naphthalenes 01 natural sciences Article Mass Spectrometry Analytical Chemistry lcsh:QD241-441 lcsh:Organic chemistry Drug Discovery medicine Red yeast rice Monacolin K [CHIM]Chemical Sciences red yeast rice Physical and Theoretical Chemistry Nuclear Magnetic Resonance Biomolecular Chromatography High Pressure Liquid ComputingMilieux_MISCELLANEOUS Detection limit Biological Products Chromatography 010405 organic chemistry Chemistry monacolins 010401 analytical chemistry Organic Chemistry 1H-NMR UHPLC-DAD-MS 0104 chemical sciences Chemistry (miscellaneous) Dietary Supplements Proton NMR Molecular Medicine 1h-nmr Lovastatin Linear correlation medicine.drug |
| Zdroj: | Molecules Molecules, MDPI, 2020, 25 (2), pp.317. ⟨10.3390/molecules25020317⟩ Molecules, Vol 25, Iss 2, p 317 (2020) Volume 25 Issue 2 |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules25020317⟩ |
| Popis: | Red yeast rice dietary supplements (RYR DS) are largely sold in Western countries for their cholesterol-lowering/regulating effect due to monacolins, mainly monacolin K (MK), which is, in fact, lovastatin, the first statin drug on the market. 1H-NMR was used as an easy, rapid and accurate method to establish the chemical profiles of 31 RYR DS and to quantify their monacolin contents. Among all the 1H resonances of the monacolins found in RYR, only those of the ethylenic protons of the hexahydronaphthalenic ring at 5.84 and 5.56 ppm are suitable for quantification because they show no overlap with the matrix signals. The total content in monacolins per capsule or tablet determined in 28 DS (the content in 3 DS being below the limit of quantification of the method, &asymp 0.25 mg per unit dose) was close to that measured by UHPLC, as shown by the good linear correlation between the two sets of values (slope 1.00, y-intercept 0.113, r2 0.986). Thirteen of the 31 RYR DS analyzed (i.e., 42%) did not provide label information on the concentration of monacolins and only nine of the 18 formulations with an indication (i.e., 50%) actually contained the declared amount of monacolins. |
| Databáze: | OpenAIRE |
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