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Foliage symptoms on soybean resembling those of sudden death syndrome were detected in Argentina during 1991 and 1992 in the Pampas Region and during 1992 and 1993 in the Northwest Region. Isolations were made in 1999, 2000, and 2001 from soybean plants (Glycine max (L.) Merr.) showing these symptoms. Five isolates of fungi obtained from taproot tissue and blue sporulation on taproot exteriors were selected for further evaluation. These isolates were plated on potato dextrose agar supplemented with streptomycin (PDAS). Based on the spore morphology, colony growth rate, morphology and pigmentation on PDAS, and lack of microconidia (1) five isolates were identified as Fusarium solani f. sp. glycines. Soybean cvs. Ripley, Spencer, Pioneer 9492RR, and A6445 RG were inoculated in greenhouse tests with each of the isolates using toothpick and soil infestation methods for a total of six experiments. Isolate 171 provided by J. Rupe (University of Arkansas, Fayetteville) was tested as a positive control. Foliar symptoms typical of sudden death syndrome and similar to those in the field were observed 14 and 25 days, respectively, after inoculations using the toothpick and soil infestation methods. Lesions produced on leaves averaged 3.6 for all five isolates and 4 for the reference strain using a disease severity scale where: 1 = no symptoms; 2 = slight symptom development with mottling and mosaic on leaves (1 to 20% foliage affected); 3 = moderate symptom development with interveinal chlorosis and necrosis on foliage (21 to 50% foliage affected); 4 = heavy symptom development with interveinal chlorosis and necrosis (51 to 80% foliage affected); and 5 = severe interveinal chlorosis and necrosis (81 to 100% foliage affected). Noninoculated controls were symptomless. Differences in virulence were observed among the isolates. Based on disease symptoms in the greenhouse and cultural morphology on PDAS, the isolates were classified as Fusarium solani f. sp. glycines. Isolates recovered from symptomatic plants resembled Fusarium solani f. sp. glycines on PDAS and peptone/p-chloro-nitrobenzene agar amended with streptomycin, confirming Koch's postulates. Fusarium solani f. sp. glycines was recovered from 60% of inoculated plants. Reference: (1) K. W. Roy et al. Plant Dis. 81:1100,1997. |
