Hormone-sensitive lipase is a cholesterol esterase of the intestinal mucosa
| Autor: | Jacques Grober, Maria Sörhede-Winzell, Philippe Besnard, Dominique Langin, Cecilia Holm, Isabelle Zaghini, Stéphanie Lucas, Juan Antonio Contreras, Aline Mairal |
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| Přispěvatelé: | Laboratoire de Physiologie de la Nutrition, Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Unité de recherche sur les obésités, IFR 31 Louis Bugnard (IFR 31), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Toulouse [Toulouse]-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Toulouse [Toulouse]-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Cell and Molecular Biology [Uppsala], Uppsala University |
| Rok vydání: | 2002 |
| Předmět: |
Male
Diacylglycerol lipase Transcription Genetic [SDV]Life Sciences [q-bio] Hormone-sensitive lipase Biochemistry 03 medical and health sciences chemistry.chemical_compound Mice Intestinal mucosa Sterol esterase medicine Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology RNA Messenger Lipase Intestinal Mucosa Lipoprotein Molecular Biology 030304 developmental biology DNA Primers 0303 health sciences Lipoprotein lipase biology Base Sequence Chemistry 030302 biochemistry & molecular biology digestive oral and skin physiology food and beverages Cell Biology Sterol Esterase Lipids Small intestine 3. Good health Kinetics Lipoprotein Lipase medicine.anatomical_structure biology.protein Cholesteryl ester [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition |
| Zdroj: | Journal of Biological Chemistry Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2003, 278 (8), pp.6510-6515. ⟨10.1074/jbc.M208513200⟩ |
| ISSN: | 0021-9258 1083-351X |
| DOI: | 10.1074/jbc.M208513200⟩ |
| Popis: | International audience; The identity of the enzymes responsible for lipase and cholesterol esterase activities in the small intestinal mucosa is not known. Because hormone-sensitive lipase (HSL) catalyzes the hydrolysis of acylglycerols and cholesteryl esters, we sought to determine whether HSL could be involved. HSL mRNA and protein were detected in all segments of the small intestine by Northern and Western blot analyses, respectively. Immunocytochemistry experiments revealed that HSL was expressed in the differentiated enterocytes of the villi and was absent in the undifferentiated cells of the crypt. Diacylglycerol lipase and cholesterol esterase activities were found in the different segments. Analysis of gut from HSL-null mice showed that diacylglycerol lipase activity was unchanged in the duodenum and reduced in jejunum. Neutral cholesterol esterase activity was totally abolished in duodenum, jejunum, and ileum of HSL-null mice. Analysis of HSL mRNA structure showed two types of transcripts expressed in equal amounts with alternative 5′-ends transcribed from two exons. This work demonstrates that HSL is expressed in the mucosa of the small intestine. The results also reveal that the enzyme participates in acylglycerol hydrolysis in jejunal enterocytes and cholesteryl ester hydrolysis throughout the small intestine. |
| Databáze: | OpenAIRE |
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