Different Dietary Proportions of Fish Oil Regulate Inflammatory Factors but Do Not Change Intestinal Tight Junction ZO-1 Expression in Ethanol-Fed Rats
| Autor: | Hsiao Yun Wang, Suh Ching Yang, Ya Ling Chen, Hsiao Li Chuang, Hsiang Chi Peng, Man Hui Pai, Yi Wen Chien |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Article Subject Immunology Vascular Cell Adhesion Molecule-1 Inflammation medicine.disease_cause Thiobarbituric Acid Reactive Substances 03 medical and health sciences Fish Oils 0302 clinical medicine Internal medicine lcsh:Pathology TBARS medicine Animals Rats Wistar Ethanol Tight junction Chemistry Body Weight Interleukin Organ Size Cell Biology CYP2E1 Intercellular Adhesion Molecule-1 Fish oil Rats Intestines Oxidative Stress 030104 developmental biology Endocrinology Liver Zonula Occludens-1 Protein Cytokines 030211 gastroenterology & hepatology Tumor necrosis factor alpha medicine.symptom Oxidative stress lcsh:RB1-214 Research Article |
| Zdroj: | Mediators of Inflammation Mediators of Inflammation, Vol 2017 (2017) |
| ISSN: | 1466-1861 0962-9351 |
| Popis: | Sixty male Wistar rats were fed a control or an ethanol-containing diet in groups C or E. The fat compositions were adjusted with 25% or 57% fish oil substituted for olive oil in groups CF25, CF57, EF25, and EF57. Hepatic thiobarbituric acid-reactive substance (TBARS) levels, cytochrome P450 2E1 protein expression, and tumor necrosis factor- (TNF-)α, interleukin- (IL-) 1β, IL-6, and IL-10 levels, as well as intracellular adhesion molecule (ICAM)-1 levels were significantly elevated, whereas plasma adiponectin level was significantly reduced in group E (p<0.05). Hepatic histopathological scores of fatty change and inflammation, in group E were significantly higher than those of group C (p<0.05). Hepatic TBARS, plasma ICAM-1, and hepatic TNF-α, IL-1β, and IL-10 levels were significantly lower, and plasma adiponectin levels were significantly higher in groups EF25 and EF57 than those in group E (p<0.05). The immunoreactive area of the intestinal tight junction protein, ZO-1, showed no change between groups C and E. Only group CF57 displayed a significantly higher ZO-1 immunoreactive area compared to group C (p=0.0415). 25% or 57% fish oil substituted for dietary olive oil could prevent ethanol-induced liver damage in rats, but the mechanism might not be related to intestinal tight junction ZO-1 expression. |
| Databáze: | OpenAIRE |
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