Potential of Non-Coding RNA as Biomarkers for Progressive Supranuclear Palsy
| Autor: | Fabio A. Simoes, Greig Joilin, Oliver Peters, Luisa-Sophie Schneider, Josef Priller, Eike Jakob Spruth, Ina Vogt, Okka Kimmich, Annika Spottke, Daniel C. Hoffmann, Björn Falkenburger, Moritz Brandt, Johannes Prudlo, Kathrin Brockmann, Franca Laura Fries, James B. Rowe, Alistair Church, Gesine Respondek, Sarah F. Newbury, P. Nigel Leigh, Huw R. Morris, Günter U. Höglinger, Majid Hafezparast |
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| Přispěvatelé: | Simoes, Fabio A [0000-0001-7630-0091], Joilin, Greig [0000-0001-5827-6382], Schneider, Luisa-Sophie [0000-0001-5822-1744], Vogt, Ina [0000-0002-2183-6937], Falkenburger, Björn [0000-0002-2387-526X], Leigh, P Nigel [0000-0002-6702-0378], Höglinger, Günter U [0000-0001-7587-6187], Apollo - University of Cambridge Repository |
| Rok vydání: | 2021 |
| Předmět: |
non-coding RNA
Down-Regulation diagnosis [Supranuclear Palsy Progressive] Article Catalysis Inorganic Chemistry progressive supranuclear palsy PSP biomarker RNA-seq Humans genetics [MicroRNAs] Physical and Theoretical Chemistry Molecular Biology Spectroscopy Supranuclear Palsy Progressive/diagnosis genetics [Supranuclear Palsy Progressive] Organic Chemistry General Medicine Computer Science Applications ddc MicroRNAs/genetics MicroRNAs ddc:540 Supranuclear Palsy Progressive Biomarkers |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 23; Pages: 14554 International journal of molecular sciences 23(23), 14554 (2022). doi:10.3390/ijms232314554 Simoes, F A, Joilin, G, Peters, O, Schneider, L-S, Priller, J, Spruth, E J, Vogt, I, Kimmich, O, Spottke, A, Hoffmann, D C, Falkenburger, B, Brandt, M, Prudlo, J, Brockmann, K, Fries, F L, Rowe, J B, Church, A, Respondek, G, Newbury, S F, Leigh, P N, Morris, H R, Höglinger, G U & Hafezparast, M 2022, ' Potential of Non-Coding RNA as Biomarkers for Progressive Supranuclear Palsy ', International Journal of Molecular Sciences, vol. 23, no. 23 . https://doi.org/10.3390/ijms232314554 |
| Popis: | Peer reviewed: True Funder: My Name’5 Doddie Foundation Funder: Marion Brownridge Funder: PSP Association Funder: National Institute for Health Research (NIHR) UCLH Clinical Research Facility, UCL Movement Disorders Centre and UCLH Biomedical Research Centre Funder: Cambridge Centre for Parkinson-plus Objective markers for the neurodegenerative disorder progressive supranuclear palsy (PSP) are needed to provide a timely diagnosis with greater certainty. Non-coding RNA (ncRNA), including microRNA, piwi-interacting RNA, and transfer RNA, are good candidate markers in other neurodegenerative diseases, but have not been investigated in PSP. Therefore, as proof of principle, we sought to identify whether they were dysregulated in matched serum and cerebrospinal fluid (CSF) samples of patients with PSP. Small RNA-seq was undertaken on serum and CSF samples from healthy controls (n = 20) and patients with PSP (n = 31) in two cohorts, with reverse transcription-quantitative PCR (RT-qPCR) to confirm their dysregulation. Using RT-qPCR, we found in serum significant down-regulation in hsa-miR-92a-3p, hsa-miR-626, hsa-piR-31068, and tRNA-ValCAC. In CSF, both hsa-let-7a-5p and hsa-piR-31068 showed significant up-regulation, consistent with their changes observed in the RNA-seq results. Interestingly, we saw no correlation in the expression of hsa-piR-31068 within our matched serum and CSF samples, suggesting there is no common dysregulatory mechanism between the two biofluids. While these changes were in a small cohort of samples, we have provided novel evidence that ncRNA in biofluids could be possible diagnostic biomarkers for PSP and further work will help to expand this potential. |
| Databáze: | OpenAIRE |
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