Apigenin decreases expression of the myofibroblast phenotype
| Autor: | Dennis A. Ricupero, Ronald H. Goldstein, Ping-Ping Kuang, David C. Rishikof, Christine F. Poliks |
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| Rok vydání: | 2001 |
| Předmět: |
Flavonoid
Biophysics macromolecular substances Biochemistry chemistry.chemical_compound IMR-90 Western blot Transforming Growth Factor beta Structural Biology Transcription (biology) Genetics medicine Humans mRNA stability RNA Messenger Apigenin Molecular Biology Protein kinase B Cells Cultured Flavonoids chemistry.chemical_classification Myofibroblast Messenger RNA α-Smooth muscle actin medicine.diagnostic_test Chemistry Muscle Smooth Cell Biology Phenotype Molecular biology α1(I) Collagen Actins Gene Expression Regulation Dactinomycin Collagen |
| Zdroj: | FEBS Letters. 506:15-21 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/s0014-5793(01)02737-5 |
| Popis: | We investigated the effect of the dietary flavonoid apigenin on myofibroblast function. We report that in myofibroblasts treated with apigenin, proliferation and basal levels of alpha1(I) collagen and alpha-smooth muscle actin mRNAs were markedly reduced. Apigenin also attenuated the transforming growth factor-beta-stimulated increases of alpha1(I) collagen and alpha-smooth muscle actin mRNAs. Characterization of the apigenin effects indicates that apigenin reduces both the stability of the alpha1(I) collagen mRNA and the rate of transcription of the alpha1(I) collagen gene through a cycloheximide-sensitive pathway. Western blot analyses indicate that Akt activity is reduced in apigenin-treated myofibroblasts. |
| Databáze: | OpenAIRE |
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