Evidence of polymorphonuclear neutrophils (PMN) activation in patients with insulin-dependent diabetes mellitus
| Autor: | H. Wysocki, Roman Klimas, Wierusz-Wysocka B, A Wykretowicz, H Siekierka, A. Szczepanik |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent Neutrophils Surface Properties Neutrophile Immunology Stimulation Biology Blood cell chemistry.chemical_compound Superoxides Internal medicine Diabetes mellitus medicine Electrochemistry Immunology and Allergy Humans Glucuronidase Superoxide Nitroblue Tetrazolium Chemotaxis Cell Biology Middle Aged medicine.disease Chemotaxis Leukocyte medicine.anatomical_structure Endocrinology Diabetes Mellitus Type 1 chemistry Female Muramidase Lysozyme Oxidation-Reduction Intracellular |
| Zdroj: | Journal of leukocyte biology. 42(5) |
| ISSN: | 0741-5400 |
| Popis: | Polymorphonuclear neutrophils' chemotaxis, surface charge, superoxide anions generation, NBT (nitro blue tetrazolium) reduction and intracellular lysozyme, and β-glucuronidase content were estimated in patients with type I diabetes mellitus in a similar state of metabolic control. The chemotaxis of diabetic cells toward bacterial chemotactic factors was similar to controls, whereas migration toward complement-derived chemoattractants was significantly reduced. Polymorphonuclear neutrophils isolated from diabetic patients, when unstimulated, produced significantly greater amounts of superoxide anions and reduced NBT more efficiently. They also revealed reduced surface charge and lower intracellular content of lysozyme, whereas β-glucuronidase content was similar to controls. The results obtained seem to indicate that neutrophils in patients with insulin-dependent diabetes manifest signs of being in the activated state. The possible mechanisms of such stimulation are discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text