Increased Human Immunodeficiency Virus (HIV) Type 1 DNA Content and Quinolinic Acid Concentration in Brain Tissues from Patients with HIV Encephalopathy
| Autor: | David E. Kleiner, Kuniaki Saito, Melvyn P. Heyes, Pim Brouwers, David A. Katz, Sean K. Stewart, Jeffrey S. Crowley, Philip A. Pizzo, Shizuko Sei |
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| Rok vydání: | 1995 |
| Předmět: |
Adult
AIDS Dementia Complex Adolescent Molecular Sequence Data Central nervous system Encephalopathy Biology Polymerase Chain Reaction Virus chemistry.chemical_compound Zidovudine Cerebellum Immunopathology medicine Humans Immunology and Allergy Child Didanosine Cells Cultured DNA Primers Brain Chemistry AIDS-Related Opportunistic Infections Base Sequence Tumor Necrosis Factor-alpha Macrophages Brain Infant Middle Aged Quinolinic Acid medicine.disease Genes pol Virology Infectious Diseases medicine.anatomical_structure Real-time polymerase chain reaction chemistry Organ Specificity Child Preschool DNA Viral Mutation Immunology HIV-1 medicine.drug Quinolinic acid |
| Zdroj: | Journal of Infectious Diseases. 172:638-647 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/172.3.638 |
| Popis: | Levels of human immunodeficiency virus type 1 (HIV-1) DNA and quinolinic acid were examined in areas of the central nervous system (CNS) and lymphoid organs (LN) from 5 AIDS patients with no clinically apparent CNS compromise (group I), 7 with CNS opportunistic diseases (group II), and 8 with HIV encephalopathy (group III). The brains from patients with HIV encephalopathy not only contained higher levels of HIV-1 DNA (cerebrum, P < .01; cerebellum, P < .05) as assessed by quantitative polymerase chain reaction but also showed a higher rate of viral pol region mutations suggestive of zidovudine or didanosine resistance than brains from patients in group I or II (P < .01). CNS quinolinic acid concentrations were significantly higher in group II and III patients than in group I (P = .03), even though quinolinic acid levels in LN were comparable among the 3 groups. These data suggest that CNS inflammatory changes associated with HIV encephalopathy may be triggered by a local productive HIV-1 infection within the CNS. |
| Databáze: | OpenAIRE |
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