Immune rejection of human dystrophin following intramuscular injections of naked DNA in mdx mice
| Autor: | Cavallini B, Halluard C, Bruce Acres, Frédéric Perraud, N. Accart, C. Thioudellet, Patrick Rodriguez, Jean-Marc Balloul, Andrea Pavirani, S. Braun, Ali-Hadji D |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
musculoskeletal diseases
congenital hereditary and neonatal diseases and abnormalities T cell Genetic enhancement Duchenne muscular dystrophy T-Lymphocytes Blotting Western Enzyme-Linked Immunosorbent Assay Injections Intramuscular Antibodies Dystrophin Mice Immune system Species Specificity Genetics medicine Animals Humans Muscle Skeletal Molecular Biology biology Genetic transfer Genetic Therapy musculoskeletal system medicine.disease Molecular biology Immunohistochemistry Mice Inbred C57BL Muscular Dystrophy Duchenne medicine.anatomical_structure Naked DNA Immunology biology.protein Mice Inbred mdx Molecular Medicine Antibody Plasmids |
| Zdroj: | Gene therapy. 7(17) |
| ISSN: | 0969-7128 |
| Popis: | Intramuscular administration of plasmid expressing full-length human dystrophin in dystrophin-deficient adult mdx mice resulted in humoral and weak specific T cell responses against the human dystrophin protein. Following plasmid injection, human dystrophin was detected in the injected muscles at 7 days, but decreased thereafter. Anti-dystrophin antibodies were found 21 days following plasmid injection, which coincided with transient myositis. This immune rejection prevented the mice from expressing human dystrophin after a second plasmid injection. No anti-DNA antibodies were found. Anti-dystrophin antibodies were seen in a smaller proportion of plasmid-injected dystrophin-competent C57BL/10 mice, suggesting that the immune rejection of dystrophin may be explained partially by species differences in the dystrophin protein. |
| Databáze: | OpenAIRE |
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