Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability
| Autor: | Felipe Riveros, Catherine Lizama, Shakeel Ahmed, Mario J. Simirgiotis, Carolina Cabalín, Jorge Bórquez, Javier Romero-Parra, Luis F Venegas-Salas, Daniel Andrade |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Antioxidant bacterioruberin Physiology medicine.medical_treatment 030106 microbiology Clinical Biochemistry cell viability in HaCaT RM1-950 Biochemistry Article high resolution MS analysis 03 medical and health sciences Pigment UHPLC medicine Viability assay Molecular Biology Carotenoid haloarchaea chemistry.chemical_classification biology carotenoids docking studies Cell Biology biology.organism_classification Orbitrap 030104 developmental biology Enzyme Haloarcula Atacama saline lake chemistry visual_art Haloarchaea visual_art.visual_art_medium Therapeutics. Pharmacology Archaea |
| Zdroj: | Antioxidants Volume 10 Issue 8 Antioxidants, Vol 10, Iss 1230, p 1230 (2021) |
| ISSN: | 2076-3921 |
| DOI: | 10.3390/antiox10081230 |
| Popis: | Haloarchaea are extreme halophilic microorganisms belonging to the domain Archaea, phylum Euryarchaeota, and are producers of interesting antioxidant carotenoid compounds. In this study, four new strains of Haloarcula sp., isolated from saline lakes of the Atacama Desert, are reported and studied by high-resolution mass spectrometry (UHPLC-Q-Orbitrap-MS/MS) for the first time. In addition, determination of the carotenoid pigment profile from the new strains of Haloarcula sp., plus two strains of Halorubrum tebenquichense, and their antioxidant activity by means of several methods is reported. The effect of biomass on cellular viability in skin cell lines was also evaluated by MTT assay. The cholinesterase inhibition capacity of six haloarchaea (Haloarcula sp. ALT-23 Haloarcula sp. TeSe-41 Haloarcula sp. TeSe-51 Haloarcula sp. Te Se-89 and Halorubrum tebenquichense strains TeSe-85 and Te Se-86) is also reported for the first time. AChE inhibition IC50 was 2.96 ± 0.08 mg/mL and BuChE inhibition IC50 was 2.39 ± 0.09 mg/mL for the most active strain, Halorubrum tebenquichense Te Se-85, respectively, which is more active in BuCHe than that of the standard galantamine. Docking calculation showed that carotenoids can exert their inhibitory activity fitting into the enzyme pocket by their halves, in the presence of cholinesterase dimers. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|