miRNA-186 improves sepsis induced renal injury via PTEN/PI3K/AKT/P53 pathway
| Autor: | Ming-Li Zhang, Feng-Qin Ren, Min Li, Wei Li |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
renal injury 030232 urology & nephrology urologic and male genital diseases Andrology Sepsis 03 medical and health sciences 0302 clinical medicine miRNA-186 Medicine PTEN Tensin Protein kinase B PI3K/AKT/mTOR pathway Kidney TUNEL assay biology business.industry apoptosis General Medicine medicine.disease 030104 developmental biology medicine.anatomical_structure Apoptosis biology.protein business Research Article |
| Zdroj: | Open Medicine Open Medicine, Vol 15, Iss 1, Pp 254-260 (2020) |
| ISSN: | 2391-5463 |
| DOI: | 10.1515/med-2020-0036 |
| Popis: | Aim The aim of this study is to explain the effects of miRNA-186 in renal injury induced by sepsis. Methods The Wistar rats were divided into 3 groups: the Sham group, Sepsis model group and the miRNA-186 group based on the model group; there were 9 rats in every group. The rat sepsis model was reproduced by cecal ligation and puncture (CLP). The rats of the miRNA-186 group were injected miRNA-186 from caudal vein. The rats of the difference group were killed after operation 24 h. The kidneys of the difference groups were taken for histopathological and cell apoptosis analysis by H&E and TUNEL assay. The relative protein expressions were measured by WB assay. miRNA-186 target to Phosphatase and tensin homologous protein (PTEN). Results Compared with the Sham group, the kidney histopathological and cell apoptosis rates of the model group were significantly damaged (P Conclusion miRNA-186 over-expression has effects that improve renal injury induced by sepsis via PTEN pathway. |
| Databáze: | OpenAIRE |
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