Phenylpropanoids and their metabolites are the major compounds responsible for blood-cell protection against oxidative stress after administration of Lippia citriodora in rats
| Autor: | Antonio Segura-Carretero, María Herranz-López, Lorena Funes, Alberto Fernández-Gutiérrez, Vicente Micol, Isabel Borrás-Linares, Rosa Quirantes-Piné |
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| Rok vydání: | 2013 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Antioxidant Neutrophils Propanols medicine.medical_treatment Glutathione reductase Anti-Inflammatory Agents Pharmaceutical Science Pharmacology Antioxidants Ferulic acid chemistry.chemical_compound Verbascoside Glucosides Phenols Drug Discovery medicine Caffeic acid Animals Rats Wistar Chromatography High Pressure Liquid chemistry.chemical_classification biology Plant Extracts Glutathione peroxidase biology.organism_classification Rats Oxidative Stress Complementary and alternative medicine chemistry Biochemistry Molecular Medicine Hydroxytyrosol Female Lippia Lemon verbena |
| Zdroj: | Phytomedicine. 20:1112-1118 |
| ISSN: | 0944-7113 |
| Popis: | Lippia citriodora (lemon verbena) has been widely used in folk medicine for its pharmacological properties. Verbascoside, the most abundant compound in this plant, has protective effects associated mostly with its strong antioxidant activity. The purpose of this study was to test the effect of L. citriodora extract intake on the antioxidant response of blood cells and to correlate this response with the phenolic metabolites found in plasma. For this purpose, firstly the L. citriodora extract was characterized and its radical scavenging activity was measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Then, catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GRed) activities were determined in lymphocytes, erythrocytes, and neutrophils isolated from rats after acute intake of L. citriodora. Phenolic metabolites were analyzed in the same plasma samples by HPLC-ESI-TOF-MS. Myeloperoxidase (MPO) activity in neutrophils, which has been proposed as a marker for inflammatory vascular damage, was also determined. After L. citriodora administration, the antioxidant enzymes activities significantly accelerated (p |
| Databáze: | OpenAIRE |
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