Tailoring copper(ii ) complexes with pyridine-4,5-dicarboxylate esters for anti-Candida activity
| Autor: | Iztok Turel, Jakob Kljun, Tina P. Andrejević, Nevena Lj. Stevanović, Ivana Aleksic, Dusan Milivojevic, Miloš I. Djuran, Marta Počkaj, Jasmina Nikodinovic-Runic, Biljana Đ. Glišić |
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| Rok vydání: | 2021 |
| Předmět: |
bakrovi(II) kompleksi
filamentacija Staphylococcus aureus Antifungal Agents Denticity Pyridines chemistry.chemical_element 010402 general chemistry Electrochemistry 01 natural sciences Medicinal chemistry Fluorescence spectroscopy Cell Line pyridine-4 5-dicarboxylates Inorganic Chemistry chemistry.chemical_compound Coordination Complexes copper(II) complexes Candida albicans DNA/BSA interakcija Pyridine udc:546.562:547.82 Humans DNA/BSA interaction protimikrobna aktivnost Bovine serum albumin Candida Cell Proliferation Gel electrophoresis antimicrobial activity biology 010405 organic chemistry Chemistry Esters DNA Copper Fluorescence 0104 chemical sciences filamentation piridin-4 5-dikarboksilati Pseudomonas aeruginosa biology.protein |
| Zdroj: | Dalton transactions, vol. 50, no. 7, pp. 2627-2638, 2021. |
| ISSN: | 1477-9234 1477-9226 |
| DOI: | 10.1039/d0dt04061d |
| Popis: | Five novel copper(II) complexes with pyridine-4,5-dicarboxylate esters as ligands, [Cu(NO$_3$)(py-2tz)(H$_2$O)$_3$]NO$_3$ (1), [Cu(NO$_3$)$_2$(py-2metz)(H$_2$O)] (2), [Cu(NO$_3$)$_2$(py-2py)(H$_2$O)]·H$_2$O (3), [CuCl$_2$(py-2tz)]$_2$ (4) and [CuCl$_2$(py-2metz)]$_n$ (5) (py-2tz is dimethyl 2-(thiazol-2-yl)pyridine-4,5-dicarboxylate, py-2metz is dimethyl 2-(4-methylthiazol-2-yl)pyridine-4,5-dicarboxylate and py-2py is dimethyl 2,2’-bipyridine-4,5-dicarboxylate), were synthesized and structurally characterized by different spectroscopic and electrochemical methods. The structure of these complexes was determined by single-crystal X-ray diffraction analysis, confirming the bidentate coordination mode of the corresponding pyridine-4,5-dicarboxylate ester to the Cu(II) ion through the nitrogen atoms. The antimicrobial potential of copper(II) complexes 1–5 was assessed against two bacterial and two Candida species. These complexes showed better growth inhibiting activity against Candida spp. with respect to the tested bacterial species, also being moderately toxic towards normal human lung fibroblast cells (MRC-5). Complexes 1 and 4 showed the greatest ability to inhibit the filamentation of C. albicans, which is an important process during fungal infection, and these two complexes efficiently inhibited the biofilm formation of C. albicans at subinhibitory concentrations. Complex 4 also successfully prevented the adhesion of C. albicans in an in vitro epithelial cell model. The mechanism of the antifungal activity of copper(II) complexes 1–5 was studied through their interaction with ct-DNA, as one of the possible target biomolecules, by fluorescence spectroscopy and gel electrophoresis. Finally, the ability of these complexes to bind to bovine serum albumin (BSA) was studied by fluorescence emission spectroscopy. |
| Databáze: | OpenAIRE |
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